炎症标志物PCT和CRP的免疫层析法双重检测任务书
2020-06-30 21:19:34
1. 毕业设计(论文)的内容和要求
论文内容主要包括绪论部分,对双重检测做一个背景介绍,描述一下pct和crp的免疫层析法双重检测的实验原理以及特点。
第二章主要讲述免疫层析法双重检测的制备过程,主要包括材料,试纸条等的制备。
第三章主要是对双重检测试纸条对pct,crp标准抗原以及实际样本的检测等作详细描述。
2. 参考文献
1. Azarpira N, Ramzi M, Aghdaie M, Daraie M (2009) Procalcitonin and C-reactive protein serum levels after hematopoietic stem-cell transplant. Exp Clin Transplant 7:115?18 2. Agnello L, Bellia C, Di Gangi M, Lo Sasso B, Calvaruso L, Bivona G, Scazzone C, Dones P, Ciaccio M (2016) Utility of serum procalcitonin and C-reactive protein in severity assessment of community-acquired pneumonia in children. Clin Biochem 49:47?0 3. Berlina AN, Taranova NA, Zherdev AV, Venqerov YY, Dzantiev BB (2013) Quantum dot-based lateral flow immunoassay for detection of chloramphenicol in milk. Anal Bioanal Chem 405:4997?000 4. Bouadma L, Luyt CE, Tubach F, Cracco C, Alvarez A, Schwebel C, Schortgen F, Lasocki S, Veber B, Dehoux M, Bernard M, Pasquet B, Regnier B, Brun-Buisson C, Chastre J, Wolff M, PRORATA trial group (2010) Use of procalcitonin to reduce patients?exposure to antibiotics Qi et al. Nanoscale Research Letters (2016) 11:167 Page 7 of 8 in intensive care units (PRORATA trial): a multicentre randomised controlled trial. Lancet 375:463?74 5. Bruchez M, Moronne M, Gin P, Weiss S, Alivisatos AP (1998) Semiconductor nanocrystals as fluorescent biological labels. Science 281:2013 6. Chan WC, Nie S (1998) Quantum dot bioconjugates for ultrasensitive nonisotopic detection. Science 281:2016?018 7. Celik LH, Demirel FG, Uras N, Oquz SS, Erdeve O, Biyikli Z, Dilmen U (2010) What are the cut-off levels for IL-6 and CRP in neonatal sepsis? J Clin Lab Anal 24:407?12 8. Cheng X, Pu X, Jun P, Zhu X, Zhu D, Chen M (2014) Rapid and quantitative detection of C-reactive protein using quantum dots and immunochromatographic test strips. Int J Nanomedicine 9:5619?626 9. Dandona P, Nix D, Wilson MF, Aljada A, Love J, Assicot M, Bohuon C (1994) Procalcitonin increase after endotoxin injection in normal subjects. J Clin Endocrinol Metab 79:1605?608 10. Eckersall PD, Conner JG, Harvie J (1991) An immunoturbidimetric assay for canine C-reactive protein. Vet Res Commun 15:17?4 11. Fu Y, Chen J, Cai B, Zhang J, Li L, Liu C, Kang Y, Wang L (2012) The use of PCT, CRP, IL-6 and SAA in critically ill patients for an early distinction between candidemia and Gram positive/negative bacteremia. J Infect 64:438?40 12. Garnacho-Montero J, Huici-Moreno MJ, Guti閞rez-Pizarraya A, L髉ez I, M醨quez-V醕aro JA, Macher H, Guerrero JM, Puppo-Moreno A (2014) Prognostic and diagnostic value of eosinopenia, C-reactive protein, procalcitonin, and circulating cell-free DNA in critically ill patients admitted with suspicion of sepsis. Crit Care 18:R116 13. Geszke-Moritz M, Moritz M (2013) Quantum dots as versatile probes in medical sciences: synthesis, modification and properties. Mater Sci Eng C Mater Biol Appl 33:1008?021 14. Hubl W, Krassler J, Zingler C, Pertschy A, Hentschel J, Gerhards-Reich C, Mack M, Demant T (2003) Evaluation of a fully automated procalcitonin chemiluminescence immunoassay. Clin Lab 49:319?27 15. Ledue TB, Weiner DL, Sipe JD, Poulin SE, Collins MF, Rifai N (1998) Analytical evaluation of particle-enhanced immunonephelometric assays for C-reactive protein, serum amyloid A and mannose-binding protein in human serum. Ann Clin Biochem 35:745?53 16. Lei Y, Xiao Q, Huang S, Xu WS, Zhang Z, He ZK, Liu Y, Deng FJ (2011) Impact of CdSe/ZnS quantum dots on the development of zebrafish embryos. J Nanopart Res 13:6895?906 17. Llorente E, Prieto B, Cardo L, Avello N, Alvarez FV (2007) Umbilical cord blood serum procalcitonin by Time-Resolved Amplified Cryptate Emission (TRACE) technology: reference values of a potential marker of vertically transmitted neonatal sepsis. Clin Chem Lab Med 45:1531?535 18. Marchesan S, Prato M (2012) Nanomaterials for (Nano)medicine. ACS Med Chem Lett 4:147?49 19. Morgenthaler NG, Struck J, Fischer-Schulz C, Seidel-Mueller E, Beier W, Bergmann A (2002) Detection of procalcitonin (PCT) in healthy controls and patients with local infection by a sensitive ILMA. Clin Lab 48:263?70 20. Murphy CJ (2002) Optical sensing with quantum dots. Anal Chem 74:520A?26A 21. Rascher D, Geerlof A, Kremmer E, Kr鋗er P, Michael S, Hartmann A, Rieger M (2014) Total internal reflection (TIRF)-based quantification of procalcitonin for sepsis diagnosis梐 point-of-care testing application. Biosens Bioelectron 59:251?58 22. Shen H, Wang H, Tang Z, Niu JZ, Lou S, Du Z, Li LS (2009) High quality synthesis of monodisperse zinc-blende CdSe and CdSe/ZnS nanocrystals with a phosphine-free method. Cryst Eng Comm 11:1733?738 23. Schwenger V, Sis J, Breitbart A, Andrassy K (1998) CRP levels in autoimmune disease can be specified by measurement of procalcitonin. Infection 26:274?76 24. Simon L, Gauvin F, Amre DK, Saint-Louis P, Lacroix J (2004) Serum procalcitonin and C-reactive protein levels as markers of bacterial infection: a systematic review and meta-analysis. Clin Infect Dis 39:206?17 25. Swanson C, D扐ndrea A (2013) Lateral flow assay with near-infrared dye for multiplex detection. Clin Chem 59:641?48 26. Volanakis JE (2001) Human C-reactive protein: expression, structure, and function. Mol Immunol 38:189?97 27. Wang W, He Y, Han Q, Chen A, Hu CM (2013) Preparation of monoclonal antibody against CRP and establishment of a quantitative ELISA for hypersensitive CRP. Immunol J 29:697?01 28. Wegner KD, Hildebrandt N (2015) Quantum dots: bright and versatile in vitro and in vivo fluorescence imaging biosensors. Chem Soc Rev 44:4792?834 29. Xu RY, Liu HW, Liu JL, Dong JH (2014) Procalcitonin and C-reactive protein in urinary tract infection diagnosis. BMC Urol 14:45 30. Yu H, Xu L, Qi XP (2014) Quantum dots labeled lateral flow strip in detecting procalcitonin in blood. Chinese J Anal Chem 42:1592?597
3. 毕业设计(论文)进程安排
2017.11.21-11.30 与导师会面,布置论文题目及要求 12.15-1.15 查阅资料,完成开题报告和任务书 2018.3.1-3.5 准备实验所需药品和器材 3.6-3.20 PCT ,CRP检测条件优化,制备标曲 3.21-4.5 多重检测条件摸索,采用间接法检测血清,全血实际样本 4.6-4.20 进行干扰实验以及稳定性检测实验 4.21-5.31 数据整理,书写论文,制作PPT 6.1-6.10 准备论文答辩
您可能感兴趣的文章
- BN嵌入型四苯并五苯:一种工具高稳定性的并五苯衍生物外文翻译资料
- MoS2和石墨烯作为助催化剂在增强的可见光光催化H2生产活性的多臂CdS纳米棒的作用外文翻译资料
- 通过在BiVO4的不同晶面上进行双助剂的合理组装制备高效率的光催化剂外文翻译资料
- 非编码RNA的固相合成研究外文翻译资料
- 氢化驱动的导电Na2Ti3O7纳米阵列作为钠离子电池阳极外文翻译资料
- 高能量及功率密度的可充电锌-二氧化锰电池外文翻译资料
- 利用导电聚合物纳米线阵列来增强电化学性能外文翻译资料
- 自支撑Na2Ti3O7纳米阵列/石墨烯泡沫和石墨烯泡沫准固态钠离子电容器电极外文翻译资料
- 基于碳纳米管金纳米粒子辣根过氧化物酶构建的过氧化氢生物传感器毕业论文
- 新型联二吡啶Pt(II)炔配合物的设计、合成及光物理性质研究毕业论文