1. 毕业设计（论文）的内容和要求

1.熟悉基本的实验操作，理解实验的基本内容；2.能做到流利阅读文献，掌握文献内容；3.熟练运用各种信息平台查阅相关信息；4.了解当下有机化学方向中与生物相关的生物偶联的内容，了解生物偶联的背景以及意义；5.熟悉并掌握生物偶联的机理。

2. 参考文献

[1]Smith Mark E B,Schumacher Felix F,Ryan Chris P,Tedaldi Lauren M,Papaioannou Danai,Waksman Gabriel,Caddick Stephen,Baker James R. Protein modification, bioconjugation, and disulfide bridging using bromomaleimides.[J]. American Chemical Society. Journal,2010,1326:. [2]Chalker Justin M,Bernardes Gon#231;alo J L,Lin Yuya A,Davis Benjamin G. Chemical modification of proteins at cysteine: opportunities in chemistry and biology.[J]. Chemistry - An Asian Journal,2009,45:. [3]Lin Yuya A,Chalker Justin M,Floyd Nicola,Bernardes Gon#231;alo J L,Davis Benjamin G. Allyl sulfides are privileged substrates in aqueous cross-metathesis: application to site-selective protein modification.[J]. American Chemical Society. Journal,2008,13030:. [4]Balan Sibu,Choi Ji-Won,Godwin Antony,Teo Ian,Laborde Carlos M,Heidelberger Sibylle,Zloh Mire,Shaunak Sunil,Brocchini Steve. Site-specific PEGylation of protein disulfide bonds using a three-carbon bridge.[J]. Bioconjugate Chemistry,2007,181:. [5]Fodje M N,Al-Karadaghi Salam. Occurrence, conformational features and amino acid propensities for the pi-helix.[J]. Protein Engineering Design and Selection,2002,155:. [6]On-Yee Chan Anna,Lui-Lui Tsai Johnson,Kar-Yan Lo Vanessa,Li Gai-Li,Wong Man-Kin,Che Chi-Ming. Gold-mediated selective cysteine modification of peptides using allenes.[J]. Chemical Communications,2013,4914:. [7]Robin Mathew P,Wilson Paul,Mabire Anne B,Kiviaho Jenny K,Raymond Jeffery E,Haddleton David M,O'Reilly Rachel K. Conjugation-Induced Fluorescent Labeling of Proteins and Polymers Using Dithiomaleimides.[J]. American Chemical Society. Journal,2013,:. [8]Ekkebus Reggy,van Kasteren Sander I,Kulathu Yogesh,Scholten Arjen,Berlin Ilana,Geurink Paul P,de Jong Annemieke,Goerdayal Soenita,Neefjes Jacques,Heck Albert J R,Komander David,Ovaa Huib. On Terminal Alkynes That Can React with Active-Site Cysteine Nucleophiles in Proteases.[J]. American Chemical Society. Journal,2013,:. [9]Tedaldi Lauren M,Smith Mark E B,Nathani Ramiz I,Baker James R. Bromomaleimides: new reagents for the selective and reversible modification of cysteine.[J]. Chemical Communications,2009,43:. [10]Crich David,Krishnamurthy Venkataramanan,Hutton Thomas K. Allylic selenosulfide rearrangement: a method for chemical ligation to cysteine and other thiols.[J]. American Chemical Society. Journal,2006,1288:.

3. 毕业设计（论文）进程安排

毕业设计进程安排: 2016.3.1 进入实验室，交待相关事宜，安排实验任务； 2016.3.1-2016.3.4 查阅文献，准备开题报告； 2016.3.10 提交开题报告。

2016.3.10-2016.3.31合成试验所需原料； 2016.4.1-2016.5.29选择相应的催化剂催化反应进行，筛选条件，优化反应条件；2016.5.30-2016.6.4 实验数据整理与分析。

2016.6.4-2016.6.10 撰写论文。