论文总字数：18004字

摘 要

二、英文摘要、关键词 2

三、论文正文 4

前言 4

1.材料与方法 　6

2.结果 15

3.讨论 21

4.结论 21

5.致谢 22

6.参考文献 23

甲磺酸氨银杏内酯B大鼠胚胎-胎鼠发育毒性试验

摘要

目的：妊娠动物自胚胎着床至妊娠终止阶段静脉注射给予甲磺酸氨银杏内酯B，评价甲磺酸氨银杏内酯B对妊娠动物、胚胎及胎仔发育的影响。

方法：采用SPF级SD大鼠，于妊娠第6-15天，静脉给予甲磺酸氨银杏内酯B。设溶媒对照组、高剂量组(100mg/kg)、中剂量组(50mg/kg)、低剂量组(25mg/kg) 、阳性对照组(给予注射用环磷酰胺，2.8mg/kg)。每组动物为孕鼠24只，给药体积为0.5 ml/100g体重。实验过程中观察并记录动物行为、体征表现；于动物妊娠第0、3、6、10、13、16、20天称重并加标准食物，隔天称剩食；于妊娠第20天处死孕鼠，检测母体着床数、黄体数、活胎数、死胎数、吸收胎数等指标，并将每个孕鼠解剖所得胎鼠半数做骨骼检查，半数做内脏检查。

结果：给药期间，对每只大鼠进行了观察，未见明显的毒性症状。阳性药组动物在孕16-20天时的体重及整个孕期的母体增重、活胎率、吸收胎率、胎鼠顶臀长、胎鼠体重、胎鼠头颅骨及胸骨畸形与溶媒对照组相比有差异显著(P＜0.05)，表明环磷酰胺对孕鼠及其胎仔有一定的影响。甲磺酸氨银杏内酯B各给药组动物试验指标在试验期间与溶媒对照组相比无显著差异。

结论：在本试验条件下，甲磺酸氨银杏内酯B 25 mg/kg及其以下剂量对SD怀孕大鼠没有明显影响，对SD大鼠子代亦无明显的致畸作用。

关键字：甲磺酸氨银杏内酯B；SD大鼠；生殖毒性试验

Ginkgolide B mesylate ammonia rat embryo - fetal developmental toxicity test

ABSTRACT

Objective: Pregnant animals from embryo implantation to pregnancy termination phase ammonia mesylate administered intravenously ginkgolide B,, embryonic and fetal development impact assessment mesylate ammonia ginkgolide B on pregnancy rats.

Methods: Using SPF SD rats on days 6-15 of gestation, intravenous mesylate ammonia Ginkgolide B. Let the vehicle control group, the high-dose group (100mg/kg), middle dose group (50mg/kg), low-dose group (25mg/kg), positive control group (given injections of cyclophosphamide, 2.8mg/kg). Each group of 24 animals was pregnant rats administered volume of 0. 5 ml/100g body weight. Experiment to observe and record the behavior of animals, signs performance; 0,3,6,10,13,16,20 day gestation animals were weighed and added standard food, leftover food, said the next day; sacrificed at 20 days of gestation pregnancy rats, the number of detected maternal implantation, corpus luteum, the number of live births, stillbirths, absorbed several other indicators of fetal and pregnant rats in each half to make fetal anatomy resulting skeletal survey, half do viscera inspection.

Results: During the administration period, each rat was observed, and no obvious signs of toxicity. Positive group of animals in 16-20 days of pregnancy weight and maternal weight gain throughout pregnancy, live birth rate, fetal absorption rate, fetal crown-rump length, fetal weight, fetal malformations and skull and sternum relative to vehicle control group than there are significant differences (P lt;0.05), showed that cyclophosphamide has a certain impact on pregnant rats and their fetuses. Ginkgolide B mesylate ammonia animal testing indicators for each treatment group during the trial showed no significant difference between the vehicle control group.

Conclusion: Under this experimental condition, mesylate ammonia lactones ginkgo B 25 mg/kg and the dose of SD rats did not significantly affect the pregnancy, offspring of SD rats was also no obvious teratogenic effects

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