1. 毕业设计（论文）的内容和要求

构建希瓦氏菌代谢网络模型并结合文献数据分析代谢调控规律

2. 参考文献

赵权宇，于水燕，史吉平. 基元模式分析在生物网络与途径分析中的应用.生物工程学报，29(6),701-715, 2013. Feng, X., Xu, Y., Chen, Y., Tang, Y.J. 2012. Integrating Flux Balance Analysis into Kinetic Models to Decipher the Dynamic Metabolism of Shewanella oneidensis MR-1. Plos Computational Biology, 8(2). Klamt, S., Saez-Rodriguez, J., Gilles, E.D. 2007. Structural and functional analysis of cellular networks with CellNetAnalyzer. BMC Syst Biol, 1, 2. Klamt, S., Schuster, S., Gilles, E.D. 2002. Calculability analysis in underdetermined metabolic networks illustrated by a model of the central metabolism in purple nonsulfur bacteria. Biotechnol Bioeng, 77(7), 734-51. Klamt, S., Stelling, J., Ginkel, M., Gilles, E.D. 2003. FluxAnalyzer: exploring structure, pathways, and flux distributions in metabolic networks on interactive flux maps. Bioinformatics, 19(2), 261-9. Tang, Y., Pingitore, F., Mukhopadhyay, A., Phan, R., Hazen, T.C., Keasling, J.D. 2007. Pathway confirmation and flux analysis of central metabolic pathways in Desulfovibrio vulgaris Hildenborough using gas chromatography-mass spectrometry and Fourier transform-ion cyclotron resonance mass spectrometry. Journal of Bacteriology, 189(3), 940-949. Tang, Y.J., Hwang, J.S., Wemmer, D.E., Keasling, J.D. 2007. Shewanella oneidensis MR-1 fluxome under various oxygen conditions. Applied and Environmental Microbiology, 73(3), 718-729. Tang, Y.J., Martin, H.G., Dehal, P.S., Deutschbauer, A., Llora, X., Meadows, A., Arkin, A., Keasling, J.D. 2009. Metabolic flux analysis of Shewanella spp. reveals evolutionary robustness in central carbon metabolism. Biotechnology and bioengineering, 102(4), 1161-9. Zhao, Q.Y., Kurata, H. 2009. Maximum entropy decomposition of flux distribution at steady state to elementary modes. Journal of Bioscience and Bioengineering, 107(1), 84-89. Zhao, Q.Y., Kurata, H. 2010. Use of maximum entropy principle with Lagrange multipliers extends the feasibility of elementary mode analysis. Journal of Bioscience and Bioengineering, 110(2), 254-261.

3. 毕业设计（论文）进程安排

1）~2018.1.13 完成开题报告 2）~2018.2.28 熟悉Matlab和 CellNetAnalyzer软件 3）~2018.3.31 构建希瓦氏菌代谢网络模型并根据实验数据进行代谢通量分解 4）~2018.4.30 代谢调控机制分析 5）~2018.5.31 撰写毕业论文 6）~2018.6 毕业答辩