1. 毕业设计（论文）的内容和要求

论文内容 小肽类水凝胶的合成使用本实验室已有蛋白酶催化形成凝胶基元，然后通过分子间作用力自组装形成水凝胶，本实验需要通过发酵以及纯化等手段获得目的蛋白合成水凝胶然后对水凝胶的安全性进行考察。

论文要求 1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，美国化学学会（acs）数据库，ca，sci等检索资源上查阅小肽类水凝胶相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

2. 参考文献

[1] Dutta SPD. Temperature, pH and redox responsive cellulose based hydrogels for protein delivery[J]. International Journal of Biological Macromolecules, 2016,87: 92-101. [2] Jiang T, Mo R, Bellotti A, et al. Gel-Liposome-Mediated Co-Delivery of Anticancer Membrane-Associated Proteins and Small-Molecule Drugs for Enhanced Therapeutic Efficacy[J]. Advanced Functional Materials, 2014,24(16): 2295-2304. [3] Jiang T, Sun W, Zhu Q, et al. Furin-mediated sequential delivery of anticancer cytokine and small-molecule drug shuttled by graphene[J]. Adv Mater, 2015,27(6): 1021-1028. [4] Jiang T, Zhang Z, Zhang Y, et al. Dual-functional liposomes based on pH-responsive cell-penetrating peptide and hyaluronic acid for tumor-targeted anticancer drug delivery[J]. Biomaterials, 2012,33(36): 9246-9258. [5] Kim BJ, Oh DX, Kim S, et al. Mussel-mimetic protein-based adhesive hydrogel[J]. Biomacromolecules, 2014,15(5): 1579-1585. [6] Li Q, Barrett DG, Messersmith PB, et al. Controlling Hydrogel MechanicsviaBio-Inspired Polymer#8211;Nanoparticle Bond Dynamics[J]. ACS Nano, 2016,10(1): 1317-1324. [7] Singh M, Kundu S, Reddy MA, et al. Injectable small molecule hydrogel as a potential nanocarrier for localized and sustained in vivo delivery of doxorubicin[J]. Nanoscale, 2014,6(21): 12849-12855. [8] Tian Y, Wang H, Liu Y, et al. A peptide-based nanofibrous hydrogel as a promising DNA nanovector for optimizing the efficacy of HIV vaccine[J]. Nano Lett, 2014,14(3): 1439-1445. [9] Williams RJ, Hall TE, Glattauer V, et al. The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel[J]. Biomaterials, 2011,32(22): 5304-5310. [10] Williams RJ, Smith AM, Collins R, et al. Enzyme-assisted self-assembly under thermodynamic control[J]. Nat Nanotechnol, 2009,4(1): 19-24. [11]张琦, 张耀东. 酶启动和调控自组装超分子水凝胶[J]. 生命的化学, 2011(4):587-591. [12]钟大根, 刘宗华, 左琴华,等. 智能水凝胶在药物控释系统的应用及研究进展[J]. 材料导报, 2012, 26(11):83-88. [13]于京, 哈伟, 师彦平. 智能水凝胶双抗癌药物控释体系[J]. 化学进展, 2015, 27(11):1640-1648. [14]侯红瑞, 张平泰. 智能水凝胶在给药系统中的应用[J]. 价值工程, 2011, 30(15):321-322. [15] 陈文俊, 程怡. 智能水凝胶与新型给药系统[J]. 中华国际医药杂志, 2002(3):193-197.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.2.26-2017.3.2 初步实验，考察初步结果 2017.3.3-2017.3.9 查阅文献，设计实验方案 2017.3.10-2017.5.4 完善实验方案，系统地进行实验 2017.5.5-2017.5.31 整理实验数据，总结实验结果 2017.6.1-2017.6.15 撰写毕业论文