1. 毕业设计（论文）的内容和要求

1.毕业论文主要内容 谷胱甘肽广泛存在于动、植物中，在生物体内有着重要的作用。

机体新陈代谢产生的过多自由基会损伤生物膜，侵袭生命大分子，加快机体衰老，并诱发肿瘤或动脉粥样硬化的产生。

谷胱甘肽在人体内的生化防御体系起重要作用，具有多方面的生理功能。

2. 参考文献

[1] Chandrasekhar V, Pandey M D, Maurya S K, et al. Two-photon-absorption technique for selective detection of copper(II) ions in aqueous solution using a dansyl-pyrene conjugate[J]. Chem Asian J, 2011, 6(9): 2246-2250. [2] Yao S, Belfield K D. Two-Photon Fluorescent Probes for Bioimaging[J]. European Journal of Organic Chemistry, 2012, 2012(17): 3199-3217. [3] Xu W J, Liu S J, Zhao X, et al. Synthesis, one- and two-photon photophysical and excited-state properties, and sensing application of a new phosphorescent dinuclear cationic iridium(III) complex[J]. Chemistry, 2013, 19(2): 621-629. [4] Kim H M, Cho B R. Small-molecule two-photon probes for bioimaging applications[J]. Chem Rev, 2015, 115(11): 5014-5055. [5] Chan J, Dodani S C, Chang C J. Reaction-based small-molecule fluorescent probes for chemoselective bioimaging[J]. Nat Chem, 2012, 4(12): 973-984. [6] Nadler A, Schultz C. The power of fluorogenic probes[J]. Angew Chem Int Ed Engl, 2013, 52(9): 2408-2410. [7] Lee M H, Sharma A, Chang M J, et al. Fluorogenic reaction-based prodrug conjugates as targeted cancer theranostics[J]. Chem Soc Rev, 2018, 47(1): 28-52. [8] Hu Q, Yu C, Xia X, et al. A fluorescent probe for simultaneous discrimination of GSH and Cys/Hcy in human serum samples via distinctly-separated emissions with independent excitations[J]. Biosens Bioelectron, 2016, 81: 341-348. [9] Wu D, Sedgwick A C, Gunnlaugsson T, et al. Fluorescent chemosensors: the past, present and future[J]. Chem Soc Rev, 2017, 46(23): 7105-7123. [10] Frye S V. The art of the chemical probe[J]. Nat Chem Biol, 2010, 6(3): 159-161. [11] Kim H M, Kim B R, An M J, et al. Two-photon fluorescent probes for long-term imaging of calcium waves in live tissue[J]. Chemistry, 2008, 14(7): 2075-2083. [12] Ma K, Sai H, Wiesner U. Ultrasmall sub-10 nm near-infrared fluorescent mesoporous silica nanoparticles[J]. J Am Chem Soc, 2012, 134(32): 13180-13183. [13] Zhang J, Shibata A, Ito M, et al. Synthesis and characterization of a series of highly fluorogenic substrates for glutathione transferases, a general strategy[J]. J Am Chem Soc, 2011, 133(35): 14109-14119. [14] Jia D, Li F, Sheng L, et al. Synthesis and assembly of ultrathin film of Ni(OH)2 nanoparticles at gas/liquid interface, its high electrocatalytical oxidation toward bio-thiols and selective determination of cysteine[J]. Electrochemistry Communications, 2011, 13(10): 1119-1122. [15] Liu J, Sun Y Q, Huo Y, et al. Simultaneous fluorescence sensing of Cys and GSH from different emission channels[J]. J Am Chem Soc, 2014, 136(2): 574-577. [16] Gao X, Li X, Li L, et al. A simple fluorescent off-on probe for the discrimination of cysteine from glutathione[J]. Chem Commun (Camb), 2015, 51(45): 9388-9390. [17] Rohand T, Qin W, Boens N, et al. Palladium-Catalyzed Coupling Reactions for the Functionalization of BODIPY Dyes with Fluorescence Spanning the Visible Spectrum[J]. European Journal of Organic Chemistry, 2006, 2006(20): 4658-4663. [18] Zhang J, Wang J, Liu J, et al. Near-infrared and naked-eye fluorescence probe for direct and highly selective detection of cysteine and its application in living cells[J]. Anal Chem, 2015, 87(9): 4856-4863. [19] Anzenbacher P, Jr., Mosca L, Palacios M A, et al. Iptycene-based fluorescent sensors for nitroaromatics and TNT[J]. Chemistry, 2012, 18(40): 12712-12718. [20] Townsend D M, Tew K D, Tapiero H. The importance of glutathione in human disease[J]. Biomedicine Pharmacotherapy, 2003, 57(3-4): 145-155. [21] Lee K S, Kim T K, Lee J H, et al. Fluorescence turn-on probe for homocysteine and cysteine in water[J]. Chem Commun (Camb), 2008, (46): 6173-6175. [22] Liu B, Wang J, Zhang G, et al. Flavone-based ESIPT ratiometric chemodosimeter for detection of cysteine in living cells[J]. ACS Appl Mater Interfaces, 2014, 6(6): 4402-4407. [23] Hettiarachchi S U, Prasai B, Mccarley R L. Detection and cellular imaging of human cancer enzyme using a turn-on, wavelength-shiftable, self-immolative profluorophore[J]. J Am Chem Soc, 2014, 136(21): 7575-7578. [24] Lee D, Kim G, Yin J, et al. An aryl-thioether substituted nitrobenzothiadiazole probe for the selective detection of cysteine and homocysteine[J]. Chem Commun (Camb), 2015, 51(30): 6518-6520.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.12.23～2017.12.28 课题任务书 2017.12.28~2018.1.18 开题报告 2018.1.18~2018.2.21 文献综述、英文翻译与试验材料准备 2018.2.21~2018.4.9 设计实验方案、进行实验 2018.4.10~2018.5.2 实验 2018.5.3~2018.5.8 实验、中期答辩 2018.5.9~2018.5.30 实验、整理实验数据、毕业论文撰写 2018.5.31~2018.6.10 毕业论文撰写、答辩