1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于大肠杆菌理性代谢改造方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Nitschke M , Costa S G, Contiero J. Structure and applications of a rhamnolipid surfactant produced in soybean oil waste[J].Applied Biochemistry and Biotechnology,2010,160(7):2066-2074. [2] Zeftawy M M , Mulligan C N. Use of rhamnolipid to remove heavy metals from wastewater by micellar-enhanced ultrafiltration(MEUF)[J].Separation and PurificationTechnology,2011,77(1):120-127. [3] Wang X, Gong L, Liang S, et al. Algicidal activity of rhamnolipid biosurfactants produced by Pseudomonas aeruginosa[J].HarmfulAlgae,2005,4(2):433-443. [4] 项海,王纬,章宏梓,等.鼠李糖脂促进含油餐饮废 水的生物降解[J]. 水处理技术,2008,34(9):65 -67. [5] Zhang Q, He G, Wang J, et al. Two-stage co-hydrolysis of rice straw by Trichoderma reesei ZM4-F3 and Pseudomonas aeruginosa BSZ-07[J].Biomass and Bioenergy,2009,33(10):1464-1468. [6] 任洁，王世梅，占新华，等．生物表面活性剂产生菌的分离鉴定及碳氮源优 化[J]．环境科学学报，2009，29 ( 10 ) :2048- 2055． [7] 杨国翠，张祥胜，朱春艳，等．高效利用废弃食用油脂产表面活性剂菌株的分离和筛选[J].山东食品发酵，2011 ( 4 ) :8- 10． [8] 张祥胜，张玉辉，戴翠娥，等．基于糖显色法测定鼠李糖含量的方法研究[J] ．河南科技学院学报: 自然科学版，2012，40( 5) : 1- 4． [9] Stipcevic T, Piljac A, Piljac G. Enhanced healing of full-thickness burn wounds using di-rhamnolipid[J]. Burns,2006,32(1):24-34. [10] Rodrigues L, Banat I M, Teixeira J, et al. Biosurfactants: potential applications in medicine[J]. JournalofAntimicrobialChemotherapy,2006,57(4): 609-618. [11] Cameotra S S , Makkar R S . Recent applications of bio- surfactants as biological and immunological molecules. Current Opinion in Microbiology,2004,7(3):262 -266. [12] Yan J, Shang P, Zheng D ,et al. Effect of simulated space gravity environment on Gibberella moniliformis EZG0807 [J].CurrentMicrobiology,2012,64(5):469-476. [13] Arino S, Marchal R, Vandecasteele J P. Identification and production of a rhamnolipidic biosurfactant by a pseudomonas species[J].Applied Microbiology and Bi-otechnology,1996,45(1-2):162-168. [14] Lotfabad T B, Abassi H, Ahmadkhaniha R, et al. Structural characterization of a rhamnolipid-type biosurfactant produced by pseudomonas aeruginosa MR01: Enhancement of di-rhamnolipid proportion using gamma irradiation[J].Colloids and Surfaces B: Biointerfaces,2010,81(2):397-405. [15] Rahim R, Ochsner UA, Olvera C, Graninger M, Messner P, Lam JS, et al. Cloning and functional characterization of the Pseudomonas aeruginosa rhlC gene that encodes rhamnosyltransferase 2, an enzyme responsible for di-rhamnolipid biosynthesis. Mol Microbiol 2001;40:708#8211;18. [16] Rehm BHA, Mitsky TA, Steinbuchel A. Role of fatty acid de novo biosynthesis in polyhydroxyalkanoic acid (PHA) and rhamnolipid synthesis by pseudomonads: establishment of the transacylase (PhaG)-mediated pathway for PHA biosynthesis in Escherichia coli. Appl Environ Microbiol 2001;67:3102#8211;9. [17] Zhu K, Rock CO. RhlA converts beta-hydroxyacyl-acyl carrier protein intermediates in fatty acid synthesis to the beta-hydroxydecanoyl-beta-hydroxydecanoate component of rhamnolipids in Pseudomonas aeruginosa. J Bacteriol 2008;190:3147#8211;54. [18] Burger M, Glaser L, Burton RM. Enzymatic synthesis of a rhamnolipid by extracts of Pseudomonas aeruginosa. Fed Proc 1963;21:82. [19] Giani C，Wullbrandt D，Rothert R，et al．Pseudomonas aeruginosa and its use in a process for the biotechnological preparation of L-rhamnose．USA，5658793， 1997，8，19． [20] Abalos A，Pinazo A，Infante M R，et al．Physicochemical and antimicrobial properties of new rhamnolipids produced by Pseudomonas aeruginosa AT10 from soybean oil refinery wastes．Langmuir，2001，17( 5) : 1367- 1371． [21] Chen G，Zhu H．Pseudomonas aeruginosa lipopolysaccharide production in the presence of rhamnolipid．Colloids and Surfaces B: Biointerfaces，2005，41( 1) : 43- 48． [22] Cohen R，Exerowa D．Surface forces and properties of foam films from rhamnolipid biosurfactants．Advances in Colloid and Interface Science，2007，134: 24- 34．

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.12-2017.2 文献查阅，完成开题报告，熟悉实验仪器。

2017.3-2017.4 对菌株的摇瓶发酵进行培养基成分的优化 2017.4-2017.5 对菌株的摇瓶发酵进行发酵条件的优化 2017.5-2017.6 综合摇瓶发酵后优化条件进行发酵罐发酵