论文总字数：16158字

摘 要

Abstract-----------------------------------------------------------------------------------II

第一章 综述---------------------------------------------------------------------------1

1.1引言-----------------------------------------------------------------------------------------------1

1.2漆酶简介----------------------------------------------------------------------------------------1

1.3漆酶的固定化方法---------------------------------------------------------------------------2

1.4固定漆酶的载体-------------------------------------------------------------------------------3

1.5小结-----------------------------------------------------------------------------------------------4

第二章 实验部分----------------------------------------------------------------------5

2.1 引言----------------------------------------------------------------------------------------------5

2.2 实验材料与仪器-----------------------------------------------------------------------------6

2.2.1 材料--------------------------------------------------------------------------------------6

2.2.2 设备--------------------------------------------------------------------------------------7

2.3 实验内容---------------------------------------------------------------------------------------8

2.3.1 水热法制备Fe₃O₄--------------------------------------------------------------------8

2.3.2 磁性琼脂糖微球的制备------------------------------------------------------------8

2.3.3 磁性琼脂糖微球的活化------------------------------------------------------------8

2.3.4 固定漆酶-------------------------------------------------------------------------------8

2.3.5 载体负载量测定---------------------------------------------------------------------9

2.3.6 酶活的测定----------------------------------------------------------------------------9

2.3.7 降解率的测定-------------------------------------------------------------------------9

第三章 实验结果与讨论-----------------------------------------------------------10

3.1 Fe₃O₄的表征--------------------------------------------------------------------------------10

3.2 磁性琼脂糖微球的表征----------------------------------------------------------------11

3.3 负载量结果分析--------------------------------------------------------------------------12

3.4 酶活结果分析-----------------------------------------------------------------------------12

3.5 降解率结果分析--------------------------------------------------------------------------13

3.6 总结------------------------------------------------------------------------------------------13

第四章 结论与展望-----------------------------------------------------------------14

4.1 结论-----------------------------------------------------------------------------------------14

4.2 展望-----------------------------------------------------------------------------------------14

参考文献--------------------------------------------------------------------------------15

致谢--------------------------------------------------------------------------------------17

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