基于功能化石墨烯的酶传感器构建研究任务书
2020-05-26 20:27:54
1. 毕业设计(论文)的内容和要求
石墨烯是由碳原子堆积形成的平面晶体,其厚度仅是一个原子尺寸。石墨烯特殊的结构(单原子厚度、 sp2 杂化的原子轨道、离域的大 π 键)决定了其在 光学、电学、热学、力学等方面拥有许多优异的性能。石墨烯结构中含有大量的 π 键,层与层之间容易因为 π-π 堆积作用而相互堆叠在一 起,宏观上表现为石墨烯的团聚。较差的分散性限制了石墨烯的进一步应用,对石墨烯进行功能化,不仅可以解决石墨烯应用中遇到的这一问题,而且还可以改善石墨烯的部分性能(如负载能力、生物兼容性等)。
本课题设计通过对氧化石墨烯化学还原的同时引入聚赖氨酸(pll),合成稳定分散的氨基化石墨烯。同时,基于金纳米粒子高催化活性和优异的导电性,本课题设计通过hbtu/三乙胺交联剂将金纳米粒子固定到氨基化石墨烯表面,合成具有优异导电性和良好生物兼容性的纳米金@石墨烯复合材料。该复合材料将为酶的固定提供良好基底,为固定化酶良好的生物微环境,实现其自然构象的保持,促进酶活性中心与电极之间有效的电子传递过程。以此为基础构建具有良好检测性能的酶电化学生物传感器。
本课题选题属于分析、应用类型,难度适中,工作量较大。
2. 参考文献
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3. 毕业设计(论文)进程安排
2015.12.26-2016.3.1 查阅资料,完成开题报告和任务书
2016.3.2-2016.4.1 确定方案,学习实验过程和操作
2016.4.2-2016.5.13 独立实验,合成纳米材料并测试性能
2016.5.14-2016.6.12 数据整理,书写论文,制作PPT
2016.6.13- 修改论文并完成答辩
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