社区获得性呼吸窘迫综合症毒素在大肠杆菌中的克隆表达任务书
2020-05-20 20:08:58
1. 毕业设计（论文）的内容和要求
1．文献查阅
掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于脂肪酶产生菌产酶条件优化方面的相关文献。
2．文献阅读及综述
2. 参考文献
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[1] Review: Recombinant protein folding and misfolding in Escherichia coli [2] The novel Fh8 and H fusion partner for soluble protein expression in E.coli : acomparison with the traditional gene fusion technology [3]fusion tags for protin solubility,purification,and immunogenicity in Escherichia col: the novel Fh8 system [4] unrelated solubility-enhancing fusion partners MBP and NusA Utilize a similar mode of action [5] a support vector machine-based method for predicting the propensity of a protein to be soluble or to form inclusion body on overexpression in Escherichia coli. [6] Characteristics of an Organic Solvent-Tolerant β#8209;Fructofuranosidase from Arthrobacter arilaitensis NJEM01 and Efficient Synthesis of Prebiotic Kestose [7] Efficient enzymatic synthesis of mangiferin glycosides in hydrophilic organic solvents [8] Xueming Wu, Jianlin Chu , Bin Wu, Sen Zhang, Bingfang He* (2012)An efficient novel glycosylation of flavonoid by b-fructosidase resistant to hydrophilic organic solvents, Bioresource Technology 129 (2013) 659#8211;662 [9] Xueming Wua,b,#8727;, Jianlin Chub, Tingting Xua, Bingfang He*(2013)Isolation, identification and pharmacokinetic analysis of fructosyl puerarins from enzymatic glycosylation [10] Jianlin Chu, Xueming Wu, Bin Wu,Rui Wang, Bingfang He* (2014)Characteristics of an Organic Solvent-Tolerant β#8209;Fructofuranosidase from Arthrobacter arilaitensis NJEM01 and Efficient Synthesis of Prebiotic Kestose; J. Agric. Food Chem. 2014, 62, 5408#8722;5411 [11] Xueming Wu, Jianlin Chu, Bin Wu, Sen Zhang, Bingfang He* (2013)Efficient enzymatic synthesis of mangiferin glycosides in hydrophilic organic solvents, RSCAdv, DOI: 10.1039/c3ra42648c [12] A A Diaz, E Tomba, R Lennarson, et al. Prediction of protein solubility in Escherichia coli using logistic regression[J]. Biotechnology and Bioengineering. 2010, 105(2): 374-383 [13] Smialowski P, Doose G, Torkler P, Kaufmann S, Frishman D: PROSO II-a new method for protein solubility prediction. FEBS J 2012, 279(12):2192#8211;2200. [14] Xiaohui N, Feng S, Xuehai H, Jingbo X, Nana L: Predicting the protein solubility by integrating chaos games representation and entropy in information theory. Expert Syst Appl 2014, 41(4):1672#8211;1679. [15] Huang H, Charoenkwan P, Kao T, Lee H, Chang F, Huang W, Ho S, Shu L,Chen W, Ho S: Prediction and analysis of protein solubility using a novel scoring card method with dipeptide composition. BMC Bioinfomratics 2012, 13(17):S3. |
3. 毕业设计（论文）进程安排
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起讫日期 |
设计（论文）各阶段工作内容 |
备 注 |
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2015.12-2016.2 |
文献查阅，完成开题报告，熟悉实验仪器。 |
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2016.3-2016.4 |
社区获得性呼吸窘迫综合症毒素的克隆 |
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2016.4-2016.5 |
融合标签融合社区获得性呼吸窘迫综合症毒素表达载体的构建 |
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2016.5-2016.6 |
表达条件的优化 |
