1. 毕业设计（论文）的内容和要求

1．文献查阅 要求掌握文献查阅技能，学会运用网络资源如中国期刊网、维普数据库、elsevier电子期刊、web of science科学引文索引、pubmed数据库等检索资源,查阅关于竹节香附素a、结肠癌以及实验涉及内容相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1. Ma B, Zhu J, Zhao A, Zhang J, Wang Y, Zhang H, et al. Raddeanin A, a natural triterpenoid saponin compound, exerts anticancer effect on human osteosarcoma via the ROS/JNK and NF-κB signal pathway. Toxicol Appl Pharmacol. 2018. 353: 87-101. 2. Y.H. Teng, J.P. Li, S.L. Liu, X. Zou, L.H. Fang, J.Y. Zhou, J. Wu, S.Y. Xi, Y. Chen, Y.Y. Zhang, S. Xu, R.P. Wang, Autophagy Protects from Raddeanin A-Induced Apoptosis in SGC-7901 Human Gastric Cancer Cells, Evid Based Complement Alternat Med. 2016. 2016(2016)9406758. 3. G. Xue, X. Zou, J.Y. Zhou, W. Sun, J. Wu, J.L. Xu, R.P. Wang, Raddeanin A induces human gastric cancer cells apoptosis and inhibits their invasion in vitro, Biochem. Biophys. Res. Commun.. 2013. 439(2013)196-202. 4. C.S. Seitz, Q. Lin, H. Deng, P.A. Khavari, Alterations in NF-kappaB function in transgenic epithelial tissue demonstrate a growth inhibitory role for NF-kappaB, Proc. Natl. Acad. Sci. U.S.A.. 1998. 95(1998)2307-2312. 5. Clevers H, Nusse R. Wnt/β-catenin signaling and disease. Cell. 2012. 149(6): 1192-205. 6. Wang MJ, Wang ZQ, Wang R, Ping J, Zhou ZG, Sun XF. The elderly patients with colorectal cancer need careful multidisciplinary evaluation and optimizing comprehensive management. Int J Colorectal Dis. 2015. 30(5): 713-4. 7. Zheng ZX, Zheng RS, Zhang SW, Chen WQ. Colorectal cancer incidence and mortality in China, 2010. Asian Pac J Cancer Prev. 2014. 15(19): 8455-60. 8. Alekshun MN, Levy SB. Molecular mechanisms of antibacterial multidrug resistance. Cell. 2007. 128(6): 1037-50. 9. Niehrs C. The complex world of WNT receptor signalling. Nat Rev Mol Cell Biol. 2012. 13(12): 767-79. 10. Mao J, Wang D, Wang Z, et al. Combretastatin A-1 phosphate, a microtubule inhibitor, acts on both hepatocellular carcinoma cells and tumor-associated macrophages by inhibiting the Wnt/β-catenin pathway. Cancer Lett. 2016. 380(1): 134-43. 11. Wang Z, Li B, Zhou L, et al. Prodigiosin inhibits Wnt/β-catenin signaling and exerts anticancer activity in breast cancer cells. Proc Natl Acad Sci U S A. 2016. 113(46): 13150-13155. 12. Bilic J, Huang YL, Davidson G, et al. Wnt induces LRP6 signalosomes and promotes dishevelled-dependent LRP6 phosphorylation. Science. 2007. 316(5831): 1619-22.

3. 毕业设计（论文）进程安排

2018.12-2019.01 实验选题，完成开题报告； 2019.01-2019.02完成竹节香附素A对诱导结肠癌细胞凋亡，抑制转移，侵袭的活性研究； 2019.03-2019.04 研究竹节香附素A竹节香附素A触发线粒体凋亡的分子机制； 2019.04-2019.05 探讨竹节香附素A对Wnt/β-catenin和NF-kB信号通路的调控作用，阐述竹节香附素A诱导细胞凋亡，抑制肿瘤转移的分子机制； 2018.05-2018.06 体内接种结肠癌细胞SW480，研究竹节香附素A体内抗结肠癌的作用机制，撰写结题报告。