1. 毕业设计（论文）的内容和要求

1、 内容 在试验过程中，制定出一套试验方案，也就是具体步骤，即：1、细胞培养（换液与传代）；2、建立对乙酰氨基酚诱导肝细胞损伤模型；3、给药干预；4、mtt实验、细胞凋亡检测、dapi染色实验和线粒体膜电位检测实验；5、数据处理。

2、要求 （1）掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，sci等检索资源上查阅关于细胞凋亡的相关文献。

（2）确定实验内容，拟定科学可行的实验方案。

2. 参考文献

[1]M.A. Heneghan, A.D. Yeoman, S. Verma, A.D. Smith, M.S. Longhi, Autoimmune hepatitis, Lancet 382 (9902) (2013) 1433#8211;1444. [2] C. Wang, Y. Xia, Y. Zheng, W. Dai, F. Wang, K. Chen, J. Li, S. Li, R. Zhu, J. Yang, Q. Yin, H. Zhang, J. Wang, J. Lu, Y. Zhou, C. Guo, Protective effects of N-acetylcysteine in concanavalin A-induced hepatitis in mice, Mediators Inflamm. 2015(2015) 189785. [3]Watkins PB, Merz M, Avigan MI, Kaplowitz N, Regev A, Senior JR. The clinical liver safety assessment best practices workshop: rationale, goals, accomplishments and the future. Drug Saf 2014;37(Suppl 1):S1-S7. [4]Antoine DJ, Jenkins RE, Dear JW, Williams DP, McGill MR, Sharpe MR, et al. Molecular forms of HMGB1 and keratin-18 as mechanistic biomarkers for mode of cell death and prognosis during clinical acetaminophen hepatotoxicity. J Hepatol 2012;56: 1070-1079. [5]Ostapowicz G, Fontana RJ, Schiodt FV, Larson A, Davern TJ, Han SH, et al. Results of a prospective study of acute liver failure at 17 tertiary care centers in the United States. Ann Intern Med 2002;137:947-954. [6]Lee WM. Acetaminophen and the U.S. acute liver failure study group: lowering the risks of hepatic failure. Hepatology. 2004; 40(1):6#8211;9. [PubMed: 15239078] [7]W. Klein-Schwartz, S. Doyon, Intravenous acetylcysteine for the treatment of acetaminophen overdose, Expert Opin. Pharmacother. 12 (2011) 119#8211;130. [8]V.S. Bebarta, L. Kao, B. Froberq, R.F. Clark, E. Lavonas, M. Qi, et al., A multicenter comparison of the safety of oral versus intravenous acetylcysteine for treatment of acetaminophen overdose, Clin. Toxicol. (Phila) 48 (2010) 424#8211;430. [9]Si Qin, Chenghao Lv, Qingshan Wang, Zhibing Zheng, Xi Sun, Minyi Tang, Fangming Deng, Extraction, identification, and antioxidant property evaluation of limonin from pummelo seeds, Animal Nutrition. 4 (2018) 281e287. [10]Tian, Q.; Miller, E. G.; Ahmad, H.; Tang, L.; Patil, B. S. Nutr. Cancer 2001, 40, 180.

3. 毕业设计（论文）进程安排

2月 确定毕业设计的课题及相关资料的查找 3月 完成相关的材料准备；完成开题报告 实验方法的确定及实验条件的优化 4月 实验阶段 5月 各项指标作比较，实验数据处理 6月 毕业论文的书写 在导师指导下修改论文，完成答辩