1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、pub med等全文电子期刊等检索资源，查阅关于mitoq、生物样品前处理技术、质谱分析技术方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

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Nutrition 33, 291-296. Chandrashekar, K.N., Muralidhara, 2009. Evidence of oxidative stress and mitochondrial dysfunctions in the testis of prepubertal diabetic rats. Int J Impot Res 21, 198-206. Chen, H., Lui, W.Y., Mruk, D.D., Xiao, X., Ge, R., Lian, Q., Lee, W.M., Silvestrini, B., Cheng, C.Y., 2018. Monitoring the Integrity of the Blood-Testis Barrier (BTB): An In Vivo Assay. Methods in molecular biology 1748, 245-252. Cheng, C.Y., Wong, E.W., Lie, P.P., Li, M.W., Mruk, D.D., Yan, H.H., Mok, K.W., Mannu, J., Mathur, P.P., Lui, W.Y., Lee, W.M., Bonanomi, M., Silvestrini, B., 2011. Regulation of blood-testis barrier dynamics by desmosome, gap junction, hemidesmosome and polarity proteins: An unexpected turn of events. Spermatogenesis 1, 105-115. Cheng, Y., Chen, G., Wang, L., Kong, J., Pan, J., Xi, Y., Shen, F., Huang, Z., 2018. Triptolide-induced mitochondrial damage dysregulates fatty acid metabolism in mouse sertoli cells. Toxicology letters 292, 136-150. Clark, J., Simon, D.K., 2009. Transcribe to survive: transcriptional control of antioxidant defense programs for neuroprotection in Parkinson's disease. Antioxid Redox Signal 11, 509-528. Danielli, N.M., Trevisan, R., Mello, D.F., Fischer, K., Deconto, V.S., da Silva Acosta, D., Bianchini, A., Bainy, A.C., Dafre, A.L., 2017. Upregulating Nrf2-dependent antioxidant defenses in Pacific oysters Crassostrea gigas: Investigating the Nrf2/Keap1 pathway in bivalves. Comparative biochemistry and physiology. Toxicology pharmacology : CBP 195, 16-26. Dashdorj, A., Jyothi, K.R., Lim, S., Jo, A., Nguyen, M.N., Ha, J., Yoon, K.S., Kim, H.J., Park, J.H., Murphy, M.P., Kim, S.S., 2013. Mitochondria-targeted antioxidant MitoQ ameliorates experimental mouse colitis by suppressing NLRP3 inflammasome-mediated inflammatory cytokines. BMC medicine 11, 178. 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Mitochondria as a Source of Reactive Oxygen and Nitrogen Species: From Molecular Mechanisms to Human Health. Antioxid Redox Sign 18, 2029-2074. Gan, L., Wang, Z., Si, J., Zhou, R., Sun, C., Liu, Y., Ye, Y., Zhang, Y., Liu, Z., Zhang, H., 2018. Protective effect of mitochondrial-targeted antioxidant MitoQ against iron ion (56)Fe radiation induced brain injury in mice. Toxicology and applied pharmacology 341, 1-7. Goncalves, G.D., Semprebon, S.C., Biazi, B.I., Mantovani, M.S., Fernandes, G.S.A., 2018. Bisphenol A reduces testosterone production in TM3 Leydig cells independently of its effects on cell death and mitochondrial membrane potential. Reproductive toxicology 76, 26-34. Hao, L., Sun, Q., Zhong, W., Zhang, W., Sun, X., Zhou, Z., 2018. Mitochondria-targeted ubiquinone (MitoQ) enhances acetaldehyde clearance by reversing alcohol-induced posttranslational modification of aldehyde dehydrogenase 2: A molecular mechanism of protection against alcoholic liver disease. 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Asian journal of andrology 13, 275-280. Jeddi, F., Soozangar, N., Sadeghi, M.R., Somi, M.H., Samadi, N., 2017. Contradictory roles of Nrf2/Keap1 signaling pathway in cancer prevention/promotion and chemoresistance. DNA repair 54, 13-21. Jiang, M., Kauppila, T.E.S., Motori, E., Li, X.P., Atanassov, I., Folz-Donahue, K., Bonekamp, N.A., Albarran-Gutierrez, S., Stewart, J.B., Larsson, N.G.R., 2017. Increased Total mtDNA Copy Number Cures Male Infertility Despite Unaltered mtDNA Mutation Load. Cell Metab 26, 429- . Johnson, K.J., 2014. Testicular histopathology associated with disruption of the Sertoli cell cytoskeleton. Spermatogenesis 4, e979106. Jones, D.P., 2002. Redox potential of GSH/GSSG couple: assay and biological significance. Methods in enzymology 348, 93-112. Kim, B., Song, Y.S., 2016. Mitochondrial dynamics altered by oxidative stress in cancer. Free radical research 50, 1065-1070. Ko, E.Y., Sabanegh, E.S., Jr., Agarwal, A., 2014. Male infertility testing: reactive oxygen species and antioxidant capacity. Fertility and sterility 102, 1518-1527. Kobayashi, M., Yamamoto, M., 2006. Nrf2-Keap1 regulation of cellular defense mechanisms against electrophiles and reactive oxygen species. Advances in enzyme regulation 46, 113-140. Li, Z., Liu, X., Wang, L., Wang, Y., Du, C., Xu, S.Y., Zhang, Y.C., Wang, C.H., Yang, C.F., 2016. The role of PGC-1 alpha and MRP1 in lead-induced mitochondrial toxicity in testicular Sertoli cells. Toxicology 355, 39-48. Liu, B., Wu, S.D., Shen, L.J., Zhao, T.X., Wei, Y., Tang, X.L., Long, C.L., Zhou, Y., He, D.W., Lin, T., Wei, G.H., 2019. Spermatogenesis dysfunction induced by PM2.5 from automobile exhaust via the ROS-mediated MAPK signaling pathway. Ecotoxicology and environmental safety 167, 161-168. Liu, L., Chang, X., Zhang, Y., Wu, C., Li, R., Tang, L., Zhou, Z., 2018. Fluorochloridone induces primary cultured Sertoli cells apoptosis: Involvement of ROS and intracellular calcium ions-mediated ERK1/2 activation. Toxicology in vitro : an international journal published in association with BIBRA 47, 228-237. Ma, B., Li, X.T., Li, J., Zhang, Q., Liu, Y.H., Yang, X.J., Sun, J.J., Yao, D., Liu, L., Liu, X.X., Ying, H.J., 2014. Quantitative analysis of tenuifolin concentrations in rat plasma and tissue using LC-MS/MS: Application to pharmacokinetic and tissue distribution study. J Pharmaceut Biomed 88, 191-200. Ma, B., Qi, H.H., Li, J., Xu, H., Chi, B., Zhu, J.W., Yu, L.S., An, G.H., Zhang, Q., 2015. Triptolide disrupts fatty acids and peroxisome proliferator-activated receptor (PPAR) levels in male mice testes followed by testicular injury: A GC-MS based metabolomics study. Toxicology 336, 84-95. Miquel, E., Cassina, A., Martinez-Palma, L., Souza, J.M., Bolatto, C., Rodriguez-Bottero, S., Logan, A., Smith, R.A., Murphy, M.P., Barbeito, L., Radi, R., Cassina, P., 2014. Neuroprotective effects of the mitochondria-targeted antioxidant MitoQ in a model of inherited amyotrophic lateral sclerosis. Free radical biology medicine 70, 204-213. Nguyen, T., Nioi, P., Pickett, C.B., 2009. The Nrf2-antioxidant response element signaling pathway and its activation by oxidative stress. The Journal of biological chemistry 284, 13291-13295. Perez-Ternero, C., Werner, C.M., Nickel, A.G., Herrera, M.D., Motilva, M.J., Bohm, M., Alvarez de Sotomayor, M., Laufs, U., 2017. Ferulic acid, a bioactive component of rice bran, improves oxidative stress and mitochondrial biogenesis and dynamics in mice and in human mononuclear cells. The Journal of nutritional biochemistry 48, 51-61. Ribeiro Junior, R.F., Dabkowski, E.R., Shekar, K.C., KA, O.C., Hecker, P.A., Murphy, M.P., 2018. MitoQ improves mitochondrial dysfunction in heart failure induced by pressure overload. Free radical biology medicine 117, 18-29. Sato, T., Yokonishi, T., Komeya, M., Katagiri, K., Kubota, Y., Matoba, S., Ogonuki, N., Ogura, A., Yoshida, S., Ogawa, T., 2012. Testis tissue explantation cures spermatogenic failure in c-Kit ligand mutant mice. Proceedings of the National Academy of Sciences of the United States of America 109, 16934-16938. Su, L.L., Mruk, D.D., Cheng, C.Y., 2011. Drug transporters, the blood-testis barrier, and spermatogenesis. J Endocrinol 208, 207-223. Sun, C., Liu, X., Di, C., Wang, Z., Mi, X., Liu, Y., Zhao, Q., Mao, A., Chen, W., Gan, L., Zhang, H., 2017. MitoQ regulates autophagy by inducing a pseudo-mitochondrial membrane potential. Autophagy 13, 730-738. Trachootham, D., Lu, W., Ogasawara, M.A., Nilsa, R.D., Huang, P., 2008. Redox regulation of cell survival. Antioxid Redox Signal 10, 1343-1374. Turner, T.T., Lysiak, J.J., 2008. Oxidative stress: a common factor in testicular dysfunction. Journal of andrology 29, 488-498. Wang, C., Qi, S., Liu, C., Yang, A., Fu, W., Quan, C., Duan, P., Yu, T., Yang, K., 2017. Mitochondrial Dysfunction and Ca(2 ) Overload in Injured Sertoli Cells Exposed to Bisphenol A. Environmental toxicology 32, 823-831. Wang, Y., Guo, S.H., Shang, X.J., Yu, L.S., Zhu, J.W., Zhao, A., Zhou, Y.F., An, G.H., Zhang, Q., Ma, B., 2018. Triptolide induces Sertoli cell apoptosis in mice via ROS/JNK-dependent activation of the mitochondrial pathway and inhibition of Nrf2-mediated antioxidant response. Acta Pharmacol Sin 39, 311-327. Wani, W.Y., Gudup, S., Sunkaria, A., Bal, A., Singh, P.P., Kandimalla, R.J., Sharma, D.R., Gill, K.D., 2011. Protective efficacy of mitochondrial targeted antioxidant MitoQ against dichlorvos induced oxidative stress and cell death in rat brain. Neuropharmacology 61, 1193-1201. Wasik, U., Milkiewicz, M., Kempinska-Podhorodecka, A., Milkiewicz, P., 2017. Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis. Scientific reports 7, 44769. Westermann, B., 2010. Mitochondrial fusion and fission in cell life and death. Nature reviews. Molecular cell biology 11, 872-884. Xi, C., Peng, S., Wu, Z., Zhou, Q., Zhou, J., 2017. Toxicity of triptolide and the molecular mechanisms involved. Biomedicine pharmacotherapy = Biomedecine pharmacotherapie 90, 531-541. Xi, Y., Feng, D.Y., Tao, K., Wang, R.L., Shi, Y.J., Qin, H.Z., Murphy, M.P., Yang, Q., Zhao, G., 2018. MitoQ protects dopaminergic neurons in a 6-OHDA induced PD model by enhancing Mfn2-dependent mitochondrial fusion via activation of PGC-1 alpha. Bba-Mol Basis Dis 1864, 2859-2870. Xiao, L., Xu, X., Zhang, F., Wang, M., Xu, Y., Tang, D., Wang, J., Qin, Y., Liu, Y., Tang, C., He, L., Greka, A., Zhou, Z., Liu, F., Dong, Z., Sun, L., 2017. The mitochondria-targeted antioxidant MitoQ ameliorated tubular injury mediated by mitophagy in diabetic kidney disease via Nrf2/PINK1. Redox biology 11, 297-311. Xiao, X., Mruk, D.D., Tang, E.I., Wong, C.K., Lee, W.M., John, C.M., Turek, P.J., Silvestrini, B., Cheng, C.Y., 2014. Environmental toxicants perturb human Sertoli cell adhesive function via changes in F-actin organization mediated by actin regulatory proteins. Human reproduction 29, 1279-1291. Zhang, T.G., Ikejima, T., Li, L.Z., Wu, R.Q., Yuan, X.Y., Zhao, J., Wang, Y.M., Peng, S.Q., 2017. Impairment of Mitochondrial Biogenesis and Dynamics Involved in Isoniazid-Induced Apoptosis of HepG2 Cells Was Alleviated by p38 MAPK Pathway. Front Pharmacol 8. Zhao, G., Mo, J., Zheng, T., Li, Y., Wu, X., Huang, J., Liu, G., Huang, Z., Yu, B., 2018. Puberty exposure to cigarette smoke extract impairs adult spermatogenesis in the mouse. Reproductive toxicology 83, 8-13.

3. 毕业设计（论文）进程安排

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