1. 毕业设计（论文）的内容和要求

木质素作为地球上第二大可再生资源，是很多芳香族化合物的重要来源。

本课题以木质素为原料，筛选微生物能够降解转化木质素，合成在化工过程中有重要应用的芳香族化合物。

2. 参考文献

1. Di Gioia D, Luziatelli F, Negroni A, Ficca AG, Fava F, Ruzzi M (2010) Metabolic engineering of Pseudomonas #64258;uorescens for the production of vanillin from ferulic acid. J Biotechnol 156:309#8211;316 2. Drakou E, Koutinas M, Pantelides I, Tsolakidou M, Vyrides I (2015) Insights into the metabolic basis of the halotolerant Pseudomonas aeruginosa strain LVD-10 during toluene biodegradation. Int Biodeterior Biodegrad 99:85#8211;94 3. Graf N, Altenbuchner J (2014) Genetic engineering of Pseudomonas putida KT2440 for rapid and high-yield production of vanillin from ferulic acid. Appl Microbiol Biotechnol 98:137#8211;149 4. Hua D, Ma C, Song L, Lin S, Zhang Z, Deng Z, Xu P (2007) Enhanced vanillin production from ferulic acid using adsorbent resin. Appl Microbiol Biotechnol 74:783#8211;790 5. Li X, Yang J, Li X, Gu W, Huang J, Zhang KQ (2008) The metabolism of ferulic acid via 4-vinylguaiacol to vanillin by Enterobacter sp. P6-4 isolated from Vanilla root. Process Biochem 43:1132#8211;1137 6. Motedayen N, Ismail MB, Nazarpour F (2013) Bioconversion of ferulic acid to vanillin by combined action of Aspergillus niger K8 and Phanerochaete crysosporium ATCC 24725. Afr J Biotechnol 12:6618#8211;6624

3. 毕业设计（论文）进程安排

2019.1.10-2019.2.26 根据课题查找相关的文献，准备3000字综述，同时，熟悉实验室仪器操作。

2019.2.26-3.31。

以木质素为底物对前期实验室筛选到的微生物的催化转化活性进行测试，得到能够转化高浓度底物的菌株；对得到的产物进行鉴定。