1. 毕业设计（论文）的内容和要求

论文内容: 通过比较不同固定化方案对β-呋喃果糖苷酶的影响，得到最佳的固定化方法，在其基础上，探究固定化酶催化合成低聚果糖的工艺，得到稳定可行的催化方案。

论文要求: 1. 文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer-link全文电子期刊，中国药典2015版等检索资源，查阅国内外关于β-呋喃果糖苷酶的固定化及催化生产低聚果糖方面的相关文献。

2. 文献阅读及综述 阅读与课题相关的外文、中文文献、检测标准，了解国内外的研究动态，撰写文献综述。

2. 参考文献

[1]曹霞, 张伟, 王英锋, et al. 微生物酶法制备低聚果糖的研究[J]. 食品科学, 2000, 21(6):31-35. [2] Avigad G (1957) Enzymatic synthesis and characterization of a new trisaccharide, α-lactosyl-β-fructofuranoside. J Biol Chem 229:121#8211;129 [3] Fujita K, Hara K, Hashimoto H, Kitahata S (1990a) Purification and some properties of β-fructofuranosidase I from Arthrobacter sp. K-1. Agric Biol Chem 54:913#8211;919. [4] Fujita K, Hara K, Hashimoto H, Kitahata S (1990b) Transfructosylation catalyzed by β-fructofuranosidase I from Arthrobacter sp. K-1. Agric Biol Chem 54:2655#8211;2661. [5]Takahama A, Kuze J, Okano S, Akiyama K, Nakane T, Takahashi H, Kobayashi T(1991) Production of lactosucrose by Bacillus natto levansucrase and some properties of the enzyme.J Jpn Soc Nutr Food Sci 38:789-796. [8]Jun Yoshikawa, Seigo Amachi, Hirofumi Shinoyama, Takaaki Fujii(2008) Production of fructooligosaccharides by crude enzyme preparations of β-fructofuranosidase from Aureobasidium pullulans. Biotechnol Lett.30:535-539 [9] Han WC, Byun SH, Lee JC, Kim MH, Kang SA, Kim CH, Wha Son E, Jang KH (2007) Synthesis of lactosucrose formed by levansucrase from Pseudomonas aurantiaca. J Biotechnol 131:S113 [10] Han WC, Byun SH, Kim MH, Sohn EH, Lim JD, Um BH, Kim CH, Kang SA, Jang KH (2009) Production of lactosucrose from sucrose and lactose by a levansucrase from Zymomonas mobilis. J Microbiol Biotechnol 19:1153#8211;1160 [11] Pilgrim, A.; Kawase, M.; Ohashi, M.; Fujita, K.; Murakami,K.; Hashimoto, K. Reaction kinetics and modeling of the enzyme-catalyzed production of lactosucrose using β-fructofuranosidase from Arthrobacter sp. K-1. Biosci. Biotechnol. Biochem. 2001, 65, 758 -765. [12]Kawase, M.; Pilgrim, A.; Araki, T.; Hashimoto. K. Lactosucrose production using a simulated moving bed reactor. Chem.Eng. Sci . 2001, 56, 453 -458. [13] Kurakake M, Onoue T, Komaki T (1996) Effect of pH on transfructosylation and hydrolys is by β-fructofuranosidase from Aspergillus oryzae. Appl Microbiol Biotechnol 45:236#8211; 239

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2018.12.26-2019.1.16 初步实验，考察初步结果 2019.3.1-2019.3.30 查阅文献，设计实验方案 2019.4.1-2019.4.30 完善实验方案，系统地进行实验 2019.5.5-2019.5.29 整理实验数据，总结实验结果 2019.5.30-2019.6.6 撰写毕业论文