1. 毕业设计（论文）的内容和要求

利用实验室丰富的微生物资源，采用传统微生物筛选与基因数据库挖掘相结合的方法开发新型单加氧酶类，并对已有单加氧酶进行分子改造拓展其底物范围，扩大生物催化工具箱，最终实现高值手性精细化学品和药物中间体的生物催化合成。

要求：学生熟练掌握微生物富集培养基础知识和掌握生物催化与转化知识。

熟悉酶的定向进化和改造。

2. 参考文献

1. M. L. Mascotti, M. Juri Ayub, H. Dudek, M. K. Sanz, M. W. Fraaije, Cloning, overexpression and biocatalytic exploration of a novel Baeyer-Villiger monooxygenase from Aspergillus fumigatus Af293. AMB Express. 3, 33 (2013). 2. A. Riebel et al., Expanding the set of rhodococcal Baeyer朧illiger monooxygenases by high-throughput cloning, expression and substrate screening. Applied Microbiology and Biotechnology. 95, 1479?489 (2012). 3. A. Kohl, V. Srinivasamurthy, D. B鰐tcher, J. Kabisch, U. T. Bornscheuer, Co-expression of an alcohol dehydrogenase and a cyclohexanone monooxygenase for cascade reactions facilitates the regeneration of the NADPH cofactor. Enzyme and Microbial Technology (2017), doi:10.1016/j.enzmictec.2017.09.003. 4. Y. Qu et al., Biotransformation of Indole and Its Derivatives by a Newly Isolated Enterobacter sp. M9Z. Applied Biochemistry and Biotechnology. 175, 3468?478 (2015). 5. S. C. Hammer et al., Anti-Markovnikov alkene oxidation by metal-oxo杕ediated enzyme catalysis. Science. 358 (2017) (available at http://science.sciencemag.org/content/358/6360/215). 6. S. Bordewick, A. Beier, K. Balke, U. T. Bornscheuer, Baeyer-Villiger monooxygenases from Yarrowia lipolytica catalyze preferentially sulfoxidations. Enzyme and Microbial Technology (2017), doi:10.1016/J.ENZMICTEC.2017.09.008. 7. F. M. Ferroni, C. Tolmie, M. S. Smit, D. J. Opperman, Alkyl Formate Ester Synthesis by a Fungal Baeyer-Villiger Monooxygenase. ChemBioChem. 18, 515?17 (2017).

3. 毕业设计（论文）进程安排

2018.1.8?018.2.28 确定课题，对课题相关文献进行调研，文献查找，包括国内外研究现状，最新进展。

实验室规章制度培训，安全培训以及各种仪器的使用。

2018.3.1?018.5.30 1) 以结构不同酮类化合物、吲哚、苯甲硫醚为底物，以保存的不同菌种进行全细胞催化，用gc/hlcp分析是否有相应产物生成。