1. 毕业设计（论文）的内容和要求

小分子水凝胶就是小分子在水溶液中通过非共价键相互作用相互结合在一起形成纤维三维网状结构，具有良好的生物可降解性和生物相容性，因此可作为细胞培养良好的支架材料。

本论文通过酶促法合成水凝胶并将其运用于脂肪干细胞的培养。

本课题主要研究内容： 1、查阅文献了解水凝胶的合成及在细胞培养方面的应用。

2. 参考文献

[1]Williams R J, Hall T E, Glattauer V, et al. The in vivo performance of an enzyme-assisted self-assembled peptide/protein hydrogel.[J]. Biomaterials, 2011, 32(22):5304-10. [2]Xiao G, Christopher B W, Lei T, et al. Preparation and characterization of a novel sodium alginate incorporated self-assembled Fmoc-FF composite hydrogel[J]. Materials Science amp; Engineering C Materials for Biological Applications, 2016, 58(9):478. [3]Mie M, Oomuro M, Kobatake E. Hydrogel scaffolds composed of genetically synthesized self-assembling peptides for three-dimensional cell culture[J]. Polymer Journal, 2013, 45(5):504-508. [4]Jayawarna V, Ali M, Jowitt T, et al. Nanostructured Hydrogels for Three‐Dimensional Cell Culture Through Self‐Assembly of Fluorenylmethoxycarbonyl#8211;Dipeptides[J]. Advanced Materials, 2006, 18(5):611-614. [5]Kraehenbuehl T P, Ferreira L P. Cell-responsive hydrogel for encapsulation of vascular cells[J]. Biomaterials, 2009, 30(26):4318-24. [6]Wang H, Yang Z. Short-peptide-based molecular hydrogels: novel gelation strategies and applications for tissue engineering and drug delivery.[J]. Nanoscale, 2012, 4(17):5259-67. [7] Hauser C A, Zhang S. Designer self-assembling peptide nanofiber biological materials[J]. Chemical Society Reviews, 2010, 39(8):2780-90. [8] Toledano S, Williams R J, Vineetha Jayawarna A, et al. Enzyme-Triggered Self-Assembly of Peptide Hydrogels via Reversed Hydrolysis[J]. Journal of the American Chemical Society, 2006, 128(4):1070-1. [9] Panda J J, Mishra A, Basu A, et al. Stimuli responsive self-assembled hydrogel of a low molecular weight free dipeptide with potential for tunable drug delivery.[J]. Biomacromolecules, 2008, 9(8):2244. [10] Wang J, Wang Z, Gao J, et al. Incorporation of supramolecular hydrogels into agarose hydrogels - A potential drug delivery carrier[J]. Journal of Materials Chemistry, 2009, 19(42):7892-7896. [11] 白靖琨, 盛成乐, 张宇,等. 酶响应型肽水凝胶及应用研究进展[J]. 生物化学与生物物理进展, 2016, 43(11):1048-1060. [12] Li-Shan W, Fan L, Jaehong L, et al. Enzymatic conjugation of a bioactive peptide into an injectable hyaluronic acid-tyramine hydrogel system to promote the formation of functional vasculature[J]. Acta Biomaterialia, 2014, 10(6):2539. [13] Li S, Chen N, Zhang Z, et al. Endonuclease-responsive aptamer-functionalized hydrogel coating for sequential catch and release of cancer cells[J]. Biomaterials, 2013, 34(2):460. [14] 王贵侠, 米坤, 刘子佳,等. 自组装纳米短肽基质在肿瘤细胞三维培养中的应用[J]. 兰州大学学报(医学版), 2010, 36(1):39-43. [15]Yang Z, Liang G, Wang L, et al. Using a kinase/phosphatase switch to regulate a supramolecular hydrogel and forming the supramolecular hydrogel in vivo.[J]. Journal of the American Chemical Society, 2006, 128(9):3038.

3. 毕业设计（论文）进程安排

2017.12～2018.1 查阅、收集文献资料 2018.2～2018.3 理论学习及总体方案规划 2018.3～2018.4 合成水凝胶。

2018.4～2018.4 熟悉脂肪干细胞培养技术 2018.4～2018.5 水凝胶用于脂肪干细胞培养的研究 2018.5～2018.5 论文撰写