1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于多糖分离纯化及表征的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] [Chen J, Pang W, Shi W, Yang B, Kan Y, He Z, Hu J. Structural elucidation of a novel polysaccharide from pseudostellaria heterophylla and stimulating glucose uptake in cells and distributing in rats by oral. Molecules. 2016; 21. [2] Hu J, Pang W, chen J, Bai S, Zheng Z, Wu X. Hypoglycemic effect of polysaccharides with different molecular weight of Pseudostellaria heterophylla. BMC Complement. Altern. Med. 2013; 13. [3] Chuanlong Z, Xiaoxia Z. Effects of polysaccharides from Pseudostellaria heterophylla on exercise endurance capacity and oxidative stress in forced swimming rats. Sci. Res. Essays. 2011; 6: 2360-2365. [4] Singh V, Kumar P, Sanghi R. Use of microwave irradiation in the grafting modification of the polysaccharides - A review. Progress in Polymer Science (Oxford). 2012; 37: 340-364. [5] Raessler M. Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants. TrAC - Trends in Analytical Chemistry. 2011; 30: 1833-1843. [6] Tonova K. Separation of poly- and disaccharides by biphasic systems based on ionic liquids. Separation and Purification Technology. 2012; 89: 57-65. [7] Qureshi RN, Kok WT. Application of flow field-flow fractionation for the characterization of macromolecules of biological interest: A review. Analytical and Bioanalytical Chemistry. 2011; 399: 1401-1411. [8] Ruiz-Matute AI, Hern#225;ndez-Hern#225;ndez O, Rodr#237;guez-S#225;nchez S, Sanz ML, Mart#237;nez-Castro I. Derivatization of carbohydrates for GC and GC-MS analyses. Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences. 2011; 879: 1226-1240. [9] Persin Z, Stana-Kleinschek K, Foster TJ, Van Dam JEG, Boeriu CG, Navard P. Challenges and opportunities in polysaccharides research and technology: The EPNOE views for the next decade in the areas of materials, food and health care. Carbohydrate Polymers. 2011; 84: 22-32. [10] Serra O, Chatterjee S, Huang W, Stark RE. Mini-review: What nuclear magnetic resonance can tell us about protective tissues. Plant Science. 2012; 195: 120-124. [11] Mirhosseini H, Amid BT. A review study on chemical composition and molecular structure of newly plant gum exudates and seed gums. Food Research International. 2012; 46: 387-398. [12] Barclay T, Ginic-Markovic M, Johnston MR, Cooper PD, Petrovsky N. Analysis of the hydrolysis of inulin using real time 1H NMR spectroscopy. Carbohydrate Research. 2012; 352: 117-125. [13] Kango N, Jains SC. Production and properties of microbial inulinases: Recent advances. Food Biotechnology. 2011; 25: 1532-4249.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.4-2017.1.13 文献查阅，研究方案制定和开题报告写作 2017.1.14-2017.1.24 开题报告的修改与复核 2017.2.4-2017.2.20 实践内容的准备 2017.2.21-2017.5.10 实践 2017.5.11-2017.5.15 实践内容处理与结果分析 2017.5.16-2017.6.1 毕业论文书写