1. 毕业设计（论文）的内容和要求

稀有人参皂苷是人参中的主要功能成分，然而受限于人参的低产量和高成长周期，稀有人参皂苷的的生产远远满足不了需求。

虽然化学合成在这方面已经有了很多的研究，但是受限于糖基的连接位点不能很好的生产我们所需的人参皂苷。

因此，我们选择使用生物酶法定向合成所需的稀有人参皂苷。

2. 参考文献

[1] Tita Ritsema, Jeanine Joling Sjef Smeekens. Patterns of fructan synthesized by onion fructan:fructan 6G-fructosyltransferase expressed in tobacco BY2 cells is fructan: fructan 1-fructosyltransferase needed in onion [J].New Phytologist, 2003, 160: 61-67 [2] 李 玉 , 路 福 平 , 王 正 祥 . 功能性低聚糖合成中糖基转移酶研究进 展 [J]. 食品科学,2013,34(9):358-363 [3] Masaki Fujishima, Hideki Sakai, Keiji Ueno, et al. Purification and characterization of a fructosyltransferase from onion bulbs and its key role in the synthesis of fructo-oligosaccharides in vivo [J]. New Phytologist, NewPhytologist, 2005, 165: 513-524 [4] 毛多斌,王雪.果糖基转移酶及低聚果糖生产研究进展[J].广东化工,2009,4(36):100-102 [5] 陈利平,韩亚伟,毛多斌,等.枯草芽胞杆菌果糖基转移酶基因的克隆及其序列分析[J].河南农业大学学报,2009,4(43):432-436 [6] 唐博,崔子寅,王艺萌,等.在大肠杆菌中高效表达具有抗病毒活性的可溶性犬α7 干扰素[J].中国预防兽医学报,2013,4(35):321-324 [7] 何小妮,蒋波,王玉海,等.非对称灭活双亲原生质体融合法选育产果糖基转移酶的米曲霉新菌株的研究[J].现代食品科技,2013,29(5):993-997 [8] Hu Y, Walker S. Remarkable structural similarities between diverse glycosyltransferases.Chemistry biology, 2002, 9(12):1287~1296.4 Ross J, Li Y, Lim E, et al. Higher plant glycosyltransferases. GenomeBiol, 2001, 2(2): REVIEWS3004. [9] Keegstra K, Raikhel N. Plant glycosyltransferases. Current opinion in plant biology, 2001,4(3):219~224.6 Coutinho PM, Deleury E, Davies GJ, et al. An evolving hierarchical family classification for glycosyltransferases. Journal of molecular biology,2003, 328(2):307~317. [10] Kroon J, Souer E, Graaff A, et al.Cloning and structural analysis of the anthocyanin pigmentation locus Rt of Petunia hybrida: characterization of insertion sequences in two mutant alleles. The Plant Journal,1994, 5(1):69~80.8 Brugliera F, Holton TA, Stevenson TW, et al. Isolation and characterization of a cDNA clone corresponding to the Rt locus of Petunia hybrida. The Plant Journal, 1994, 5(1):81~92. [11] Boss PK, Davies C, Robinson SP. Expression of anthocyanin biosynthesis pathway genes in red and white grapes. Plant Molecular Biology,1996, 32(3):565~569. [12] Fukuchi-Mizutani M, Okuhara H, Fukui Y, et al. Biochemical and molecular characterization of a novel UDP-glucose: anthocyanin 3'-O-glucosyltransferase, a key enzyme for blue anthocyanin biosynthesis, from gentian. Plant physiology, 2003, 132(3):1652. [13] Jones P, Vogt T. Glycosyltransferases in secondary plant metabolism: tranquilizers and stimulant controllers. Planta, 2001, 213(2):164~174. [14] Poppenberger B, Berthiller F, Lucyshyn D, et al. Detoxification of the Fusarium mycotoxin deoxynivalenol by a UDP #8211;glucosyltransferase from Arabidopsis thaliana. Journal of Biological Chemistry,2003, 278(48):47905~47914. [15] Zagrobelny M, Bak S, Rasmussen AV, et al. Cyanogenic glucosides and plant-insect interactions. Phytochemistry, 2004, 65(3):293~306. [16] Beveridge CA, Murfet IC, Kerhoas L, et al. The shoot controls zeatin riboside export from pea roots. Evidence from the branching mutant rms4. The Plant Journal, 1997, 11(2):339~345. [17] Staswick PE, Tiryaki I. The oxylipin signal jasmonic acid is activated by an enzyme that conjugates it to isoleucine in Arabidopsis.The Plant Cell, 2004, 16(8):2117. [18] Bowles D, Isayenkova J, Lim EK, et al. Glycosyltransferases: managers of small molecules.Current opinion in plant biology,2005, 8(3):254~263. [19] Hughes J, Hughes MA. Multiple secondary plant product UDP-glucose glucosyltransferase genes expressed in cassava (Manihot esculenta Crantz) cotyledons. Mitochondrial DNA, 1994,5(1):41~49. [20] Shao H, He X, Achnine L, et al. Crystal structures of a multifunctional triterpene/flavonoid glycosyltransferase from Medicago truncatula.The Plant Cell Online, 2005, 17(11):3141~3154.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.12---期末 文献查阅，完成开题报告，熟悉仪器 2017.3---2017.4 确定克隆表达的酶及来源菌株 2017.4---2017.5 进行酶的克隆表达及优化发酵 2017.5---2017.6 确定找到的糖基转移酶的功能 2017.6.2---2017.6.10 撰写毕业论文