1. 毕业设计（论文）的内容和要求

脂肪酶的固定化是提高酶的催化稳定性的重要方法，开发新型的交联剂用于酶的固定化具有重要的实际应用价值。

论文对葡聚糖进行醛基修饰，用于脂肪酶的固定化，通过对修饰工艺的优化研究，考察醛基葡聚糖用于交联脂肪酶的效果，为固定化脂肪酶的应用提供实验支撑。

2. 参考文献

[1]付海霞,杨国龙,毕艳兰,孙尚德. 脂肪酶修饰研究进展[J]. 粮食与油脂, 2013 (1) :1-4. [2] CH Kuo, YC Liu, CMJ Chang, JH Chen, C Chang. Optimum conditions for lipase immobilization on chitosan-coated Fe 3 O 4 nanoparticles [J]. Carbohydrate Polymers , 2012 , 87 (4) :2538-254. [3] Q Q Cai, C B Hu, N Yang, et al. Enhanced activity and stability of industrial lipases immobilized onto spherelike bacterial cellulose[J]. International Journal of Biological Macromolecules, 2018, 1174-1181. [4] W L Xie, M Y Huang. Immobilization of Candida rugosa lipase onto graphene oxide Fe3O4 nanocomposite: Characterization and application for biodiesel production[J]. Energy Conversion and Management, 2018, 42-53. [5] Y Bi, H Zhou, H Jia, P Wei. Polydopamine-mediated preparation of an enzyme-immobilized microreactor for the rapid production of wax ester [J]. Rsc Advance , 2017 , 7 (20) :12283-12291. [6]王宁. 聚合物改性的纳米载体固定化脂肪酶的研究[D]. 安徽工程大学.2018. [7] H B Suo, Z Gao, L L Xu, et al. Synthesis of functional ionic liquid modified magnetic chitosan nanoparticles for porcine pancreatic lipase immobilization[J]. Materials Science Engineering C, 2019, 356-364. [8] Ali Z，Tian L，Zhao P，et al．Immobilization of lipase on mesoporous silica nanoparticles with hierarchical fibrous pore[J]．Journal of Molecular Catalysis B：Enzymatic 2016，134，129-135． [9] Z Jiang, M Yu, L Ren, H Zhou, P Wei. Synthesis of phytosterol esters catalyzed by immobilized lipase in organic media[J]. Chinese Journal of Catalysi , 2013 , 34 (12) :2255-2262. [10] L Ren, H Jia, M Yu, W Shen, H Zhou. Enhanced catalytic ability of Candida rugosa lipase immobilized on pore-enlarged hollow silica microspheres and cross-linked by modified dextran in both aqueous and non-aqueous phases[J]. Biotechnology Bioprocess Engineering , 2013 , 18 (5) :888-896. [11] Y Bi, M Yu, H Zhou, H Zhou, P Wei. Biosynthesis of oleyl oleate in solvent-free system by Candida rugosa Lipase (CRL) immobilized in macroporous resin with cross-linking of aldehyde-dextran[J]. Journal of Molecular Catalysis B Enzymati , 2016 , 133 :1-5.

3. 毕业设计（论文）进程安排

2019.1.3-2019.2.28 查阅文献，制订实验方案，完成开题报告 2019.3.1-2019.5.31 实验研究 2019.6.1-2019.6.10 数据整理分析，撰写毕业论文 2019.6.11-2019.6.20 论文修改，完成论文答辩