论文总字数：17484字

摘 要

背景 勃起功能障碍（Erectile dysfunction，ED）是糖尿病（Diabetes mellitus,DM）非常普遍的一种并发的症状。糖尿病人群和一般人群相比较的话要更容易出现勃起功能障碍。数据显示一般人群患勃起功能障碍的几率大概只有DM患者的三分之一,DM患者的患勃起功能障碍的几率高达百分之五十到百分之九十,同时DM患者出现ED的年龄也比一般人群要小,还有一般人群中存在ED的情况，但是他们的症状会比糖尿病人轻微。糖尿病性勃起功能障碍主要是因为血管等器官组织由于糖尿病而产生的变异所引起的。想要有效的治疗糖尿病性勃起功能障碍，那么就要有效的控制体内的血糖、血压与血脂等指标。想要更加彻底的了解糖尿病性勃起功能障碍(DMED),就需要创造一个合适的DMED动物模型。

方法 把SD雄性大鼠随机分为糖尿病组与正常组。糖尿病组使用用60 mg/kg剂量腹腔注射链脲佐菌素（STZ）。正常组,腹腔注射柠檬酸钠-柠檬酸缓冲液。四天之后,检查大鼠体内的血糖含量,在这以后每个星期都要检查血糖1次。8周后,通过进行观察大鼠扑捉行为学情况、测定大鼠海绵体内压及性腺指数，检测大鼠血清睾酮含量来验证大鼠糖尿病勃起功能障碍模型。

结果 糖尿病组大鼠和正常组相比较，血糖有很明显的增加（P＜0.01）；扑捉的次数有着明显的降低与此同时潜伏期也明显的变长；最大海绵体内压（ICP）与平均颈动脉压（MAP）的比值显著下降（P＜0.01）；性腺指数显著下降；血清睾酮含量也有显著下降（P＜0.01）。

结论　以60mg/kg腹腔注射STZ可诱导大鼠出现典型的糖尿病临床症状,血糖明显增高,形成糖尿病，8周后糖尿病大鼠勃起功能明显受损，可形成糖尿病性勃起功能障碍模型。

关键词 勃起功能障碍；糖尿病；链脲佐菌素；SD大鼠

An Experimental Study on the Establishment of Diabetic model of erectile dysfunction in rats

Abstract

Background Erectile dysfunction (ED) is a common complication of diabetes (Diabetes mellitus, DM).People with diabetes have a greater risk of erectile dysfunction than the general population.The data show that the general population suffering from erectile dysfunction is probably only one-third of DM patients,DM patients with erectile dysfunction risk of up to 50 percent to 90 percent,At the same time the age of DM patients is younger than the general population, and the general population with ED symptoms of people should be relatively light.Diabetic erectile dysfunction is caused by diabetes-induced changes in blood vessels, neuropathy, and muscle tissue.Control of blood sugar, blood pressure and blood lipids are the basis for the treatment of diabetic erectile dysfunction.To further study diabetes mellitus erectile dysfunction (DMED),it is necessary to establish a stable and reliable DMED animal model.

Methods SD male rats were randomly divided into diabetic group and normal group.Diabetic rats were injected intraperitoneally with streptozotocin (STZ) at a dose of 60 mg / kg.Rats in the normal group were injected with citric acid - citrate buffer intraperitoneally.Four days later, the rats' blood glucose was measured,after each week to measure blood sugar 1 times.After 8 weeks, the rats were sacrificed and the gonadal index was measured by observing the behavior of rats.the rat serum testosterone was tested to verify the model of diabetic erectile dysfunction.

Results Compared with the normal control group,the blood glucose of diabetic rats was significantly increased (P lt;0.01);the times of captures was significantly reduced and the incubation period was significantly prolonged;The ratio of maximal cavernous pressure (ICP) to mean carotid artery pressure (MAP) was significantly decreased (P lt;0.01);gonadal index decreased significantly;Serum testosterone levels also decreased significantly (P lt;0.01).

Conclusion Intraperitoneal injection of STZ at 60 mg / kg induced typical clinical symptoms of diabetes in rats,blood sugar was significantly increased,the formation of diabetes,After 8 weeks, the erectile function of diabetic rats was significantly impaired,can form a model of diabetic erectile dysfunction.

Key words： Erectile dysfunction; diabetes; streptozotocin ; SD rats

目 录

摘要 I

ABSTRACT II

第一章 文献综述 4

1.1引言 1

1.1.1影响勃起的器质性因素 1

1.1.2 影响勃起功能的心理因素 1

1.2 糖尿病性勃起功能障碍概述 2

1.2.1用于相关研究的糖尿病动物模型 2

1.3 国内外研究状况 3

1.4 课题设计思路 5

第二章 材料与方法 6

2.1 材料与方法 6

2.1.1 主要试剂与仪器 6

2.2 实验动物 6

2.3 糖尿病大鼠模型的建立 6

2.3.1 建立糖尿病大鼠的方法 6

2.3.2 STZ注射剂量的选择 7

2.3.3血糖与体重的测定 7

2.3.4对大鼠行为学观察 7

2.4 大鼠ICP及MAP检测 8

2.5性腺指数的测量 8

2.6睾酮含量测定 8

2.7统计学分析 8

第三章 结果 10

3.1 造模 10

3.2 体重与血糖情况 10

3.3 大鼠行为学观察 10

3.4 大鼠内压检测 11

3.5 性腺指数情况 11

3.6 睾酮含量情况 12

第四章 讨论 14

参考文献 15

致 谢 18

第一章 文献综述

1.1引言

勃起功能障碍（Erectile dysfunction，ED）又可以叫为阳痿。是指阴茎持续不能达到或

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