1. 毕业设计（论文）的内容和要求

青霉素酰化酶具有良好的催化酰化/去酰化特性，因此在医药工业中发挥着重要的作用，主要应用于半合成青霉素和头孢菌素类的工业生产，可以催化制备高效、广谱、适用于不同用途的新型β-内酰胺抗生素。

尽管青霉素酰化酶的研究以开展多年并取得重要进展，但野生型青霉素酰化酶的性能仍然难以满足需求，我们需要深入研究青霉素酰化酶结构，从而可以为高性能酶的改造与进一步开发奠定基础，这对酶法合成β-内酰胺类抗生素具有重大意义。

本课题主要研究内容： 本文从实验室自行筛选的achromobacter xylosoxidans来源的青霉素酰化酶pgapx02出发，对pgapx02进行定点饱和突变，筛选得到高效合成氨苄西林的突变子，并对合成条件进行优化。

2. 参考文献

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3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.12～2018.1 查阅、收集文献资料 2018.3～2018.3 理论学习及总体方案规划 2018.3～2018.4 对PGApx02的βF24位点进行定点饱和突变 2018.4～2018.4 筛选在氨苄西林合成中效果较好的突变子 2018.4～2018.5 优化突变子的合成条件 2018.5～2018.5 论文撰写