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摘要：酪丁酸梭菌可以利用葡萄糖、木糖、纤维二糖、阿拉伯糖等多种底物进行产酸发酵，是一种适于木质纤维素同步糖化发酵产酸的菌种。本次设计针对于氧气对酪丁酸梭菌的影响，进行它的分子改造。通过配制培养基RCM、培养皿，对改造后的菌种进行连续培育，通过培养皿中菌落量判断活性。然后进行一系列的转接、提取、萃取，最后利用气相色谱来进一步判断提高其耐氧化性能的程度。

关键词：酪丁酸梭菌； 分子改造； 耐氧化； 培养

Abstract: Clostridium tyrobutyricum can use acid, glucose, xylose, fiber two sugar, Arabia sugar and other substrates to produce acid fermentation. This design is aimed at the effect of oxygen on Clostridium tyrobutyricum and its molecular modification. Through the preparation of culture medium RCM and Petri dishes, the transformed strains were cultured continuously, and the activity was determined by the amount of bacteria in the culture dish. Then a series of transfer, extraction, extraction, and further determine the degree of oxidation resistance.

Key words：Clostridium tyrobutyricum； Molecular modification； Resistance to oxidation； culture