论文总字数：19581字

摘 要

本文重点研究了红掌组织培养的条件以及对组织培养红掌的方法的优化。通过设计不同的培养基分别对红掌进行组织培养，以及对组织培养条件进行单因素对比试验，确定对红掌组织培养有促进作用的物质和方法，如MS培养基的浓度，NAA、2，4-D、6-BA对红掌组织培养的影响和最佳浓度，同时对组织培养过程中pH浓度和光照强度进行了研究，另外，针对研究过程中出现的大量褐化的情况，在培养基中分别加入活性炭和抗生素，并做了对比研究，为以后进一步研究做好准备工作。

关键词：红掌 优化 组织培养

The optimization of micropropagation of Authurium andraeanum

Abstract

This article is mainly about the method of micropropagation of Authurium andraeanum and the optimization of it. Design different kinds of media and culture the Authurium andraeanum by these different selection media. And do research of the tissue culture by single factor experiment, find out the method and discover the substance which can promote the growth of the plants, such as concentration of the Murashige and Skoog medium, the influence of NAA, 2,4-D, 6-BA and the best concentration of these matter. In the meantime, the pH and light control was studied. The activated carbon and antibiotic was added in the media to figure out the problem of browning.

Key words: Authurium andraeanum; optimization; tissue culture

目录

摘要····························································································Ⅰ

Abstract ········· ·········································································· Ⅱ

第一章 文献综述 ······································································· 1

1.1 红掌基本概述 ····· ···················· ··················································1

1.1.1红掌的品种及介绍 ················································ ················1

1.1.2红掌的生物学特征 ·······························································1

1.1.3红掌的传统繁殖技术····························································· 2

1.1.4红掌的引种历史和现状 ························································· 2

1.2红掌组织培养繁殖技术································································· 4

1.2.1红掌组织培养研究进展 ··························································4

1.2.2红掌组织培养的发生途径 ······················································7

1.2.3外植体的选择 ·····································································7

1.2.4基本培养基对红掌组织培养植株再生体系的响···························· 8

1.2.5 碳源对红掌离体再生培养的影响 ·············································8

1.2.6 外源激素对红掌组织培养植株再生体系的影响··························· 8

1.2.7 光照条件对离体培养的影响····················································9

1.3 关于本文的研究内容和意义···························································9

第二章 实验材料与方法······························································10

2.1实验材料、主要仪器和使用的试剂·················································10

2.2实验方法···················································································11

2.2.1外植体预处理，以及外植体种类对愈伤组织发生的影响 ··············12

2.2.2 碳源种类以及碳源浓度对诱导愈伤组织的影响···························12

2.2.3 不同激素配比对红掌叶片愈伤组织诱导的影响···························13

2.2.4 不同添加物对红掌愈伤组织诱导及褐化的影响试验·····················14

2.2.5 光照强度对红掌愈伤组织诱导的影响·······································14

第三章 结果与数据分析································································15

3.1外植体取材部位的影响结果 ························································ ·· 15

3.2 碳源对愈伤组织诱导的影响··························································· 15

3.3 不同激素配比对诱导愈伤组织的影响 ···············································16

3.4 不同抗褐化添加物对诱导愈伤组织以及褐化的影响 ·····························17

3.5 不同光照强度对诱导愈伤组织的影响 ···············································19

第四章 结论与展望·······································································20

4.1本文通过对红掌培养条件的研究与优化得出以下结论 ·························20

4.2 展望·· ·······················································································20

参考文献 ······ ··············································································21

第一章 文献综述

请支付后下载全文，论文总字数：19581字