1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于甲醇蛋白方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

1.Heggeset TMB, Krog A, Balzer S, Wentzel A, Ellingsen TE, Brautaset T (2012) Genome sequence of thermotolerant Bacillus methan olicus: features and regulation related to methylotrophy and production of l-lysine and L-glutamate from methanol. Appl Environ Microbiol 78(15):5170 2. Jakobsen OM, Benichou A, Flickinger MC, Valla S, Ellingsen TE, Brautaset T (2006) Upregulated transcription of plasmid and chromosomal ribulose monophosphate pathway genes is critical for methanol assimilation rate and methanol tolerance in the methylotrophic bacterium Bacillus methanolicus. J Bacteriol 188:3063#8211;3072. doi:10.1128/JB.188.8.3063-3072.2006 3. Komives CF, Cheung LY, Pluschkell SB, Flickinger MC (2005) Growth of Bacillus methanolicus in seawater-based media. J Ind Microbiol Biotechnol 32:61#8211;66. doi:10.1007/ s10295-004-0195-9 4. Kuwahara H (1980) Metabolism of lignin-related compounds by bacteria in lignin biodegradation. In: Kirk TK, Higuchi T, ChangH (eds) Lignin biodegradation: microbiology, chemistry and potential applications. CRC Press, New York 5. Lee GH, Hur W, Bremmon CE, Flickinger MC (1996) Lysine production from methanol at 50 #176;C using Bacillus methanolicus: modeling volume control, lysine concentration, and productivity using a three-phase continuous simulation. Biotechnol Bioeng 49:639#8211;653. doi:10.1002/(SICI)1097-0290(19960320)49:63.0.CO;2-P 6. Lidstrom ME (2006) Aerobic methylotrophs. In: Dworkin M, Falkow S, Rosenberg E, Schleifer K, Stackebrandt E (eds) The prokaryotes: a handbook on the biology of bacteria. Springer,New York 7. Lindner R (2007) Construction of an airlift bioreactor and comparison with STR for fed-batch growth of Bacillus methanolicus on methanol. In: Diploma Thesis, Technische Fach Hochschule,Berlin 8. Mozzanega P (2010) Characterization of the enzymes involved in methanol dissimilation in Bacillus methanolicus: assessment of enzyme-based biohydrogen production for a cell-free biofuel cell. University of Bath, England 9. M#252;ller JEN, Litsanov B, Bortfeld-Miller M, Trachsel C, GrosBeck, D.A., Kalyuzhnaya, M.G., Malfatti, S., Tringe, S.G., Glavina Del Rio, T., Ivanova,N., Lidstrom, M.E. shistose rdova, L., 2013. 10.A metagenomic insight into freshwater methane-utilizing communities and evidence for cooperation between the Methylococcac eae and the Methylophilaceae. PeerJ 1, e23. 11. Brettin, T., Davis, J.J., Disz, T., Edwards, R.A., Gerdes, S., Olsen, G.J., Olson, R., Overbeek, R., Parrello, B., Pusch, G.D., Shukla, M., Thomason Iii, J.A., Stevens, R., Vonstein, V., Wattam, A.R., Xia, F., 2015. RASTtk: a modular and extensible implement ation of the RAST algorithm for building custom annotation pipelines and annotating batches of genomes. Sci. Rep. 5, 8365. 12. Chistoserdo va, L., 2011. Modulirity of methylotrophy, revisited. Environ. Microbiol. 13, 2603#8211;2622. 13. Chistoserdova, L., Lapidus, A., Han, C., Goodwin, L., Saunders, L., Brettin, T., Tapia, R., Gilna, P., Lucas, S., Richardson, P.M., 200 7. Genome of Methylobacillus flagellatus, molecular basis for obligate methylotrophy, and polyphyletic origin of methylotrophy. J. Bacteriol. 189, 4020#8211;4027. 14.Goenrich, M., Thauer, R., Yurimoto, H., Kato, N., 2005. Formaldehyde activating enzyme (Fae) and hexulose-6-phosphate synthase (Hps) in Methanosarcina barkeri: a possible function in ribose-5-phosphate biosynthesis. Arch. Microb iol. 184, 41#8211;48. 15. He, R., Wooller, M.J., Pohlman, J.W., Catranis, C., Quensen, J., Tiedje, J.M., Leigh, M.B.,2012. Identification of functionally active aerobic methanotrophs in sediments from an arctic lake using stable isotope probing. Environ. Microbiol. 14,1403#8211;1419.

3. 毕业设计（论文）进程安排

2017.12-2018.2 文献查阅，完成开题报告，熟悉实验仪器。

2018.3-2018.4 选择合适培养基，筛出能够利用高浓度甲醇的甲基型菌株。

2018.4-2018.5 对筛出的几株菌，做种属鉴定。