1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解糙皮侧耳发酵产麦角硫因的提纯方法及过程，并验证其抗氧化功能，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]．Akanmu D, Cecchini R, Aruoma O, et al.. The Antioxidant Action of Ergothioneine [J]. Archives of Biochemistry and Biophysics, 1991, 288(1):10-16. [2]．Aruoma O I, Whiteman W, England T G, et al..Antioxidant Action of Ergothioneine: Assessment of Its Ability to Scavenge Peroxynitrite[J]. Biochemical and Biophysical Research Communication, 1997,231(2):389-391. [3]．Bao H N D, Ushio H, Ohshima T. Antioxidative Activity and Antidiscoloration Efficacy of Ergothioneine in Mushroom (Flammulina velutipes) Extract Added to Beef and Fish Meats[J]. Journal of Agricultural and Food Chemistry, 2008,56(21):10032-10040. [4]．Bao H N D, Ushio H, Ohshima T. Antioxidative Activities of Mushroom (Flammulina velutipes) Extract Added to Bigeye Tuna Meat: Dose-Dependent Efficacy and Comparison with Other Biological Antioxidants[J]. Journal of Food Science, 2009,74(2):C162-C169. [5]．Bao H N, Osako K, Ohshima T. Value-added use of mushroom ergothioneine as a colour stabilizer in processed fish meats[J]. Journal of the Science of Food and Agriculture, 2010,90(10):1634-1641. [6]．Botta C, Di Giorgio C, Sabatier A, et al.. Genotoxicity of visible light (400#8211;800nm) and photoprotection assessment of ectoin, l-ergothioneine and mannitol and four sunscreens[J]. Journal of Photochemistry and Photobiology B: Biology, 2008,91(1):24-34. [7]．Cardoso-Ugarte G A, Sosa-Morales M E, Ballard T, et al.. Microwave-assisted extraction of betalains from red beet (Beta vulgaris)[J]. LWT - Food Science and Technology, 2014,59(1):276-282. [8]．Cheah I K, Halliwell B. Ergothioneine; antioxidant potential, physiological function and role in disease[J]. Biochimica et Biophysica Acta (BBA) #8211;Molecular Basis of Disease, 2012,1822(5):784-793. [9]．Chen S, Ho K, Hsieh Y, et al.. Contents of lovastatin, γ-aminobutyric acid and ergothioneine in mushroom fruiting bodies and mycelia[J]. LWT-Food Science and Technology, 2012,47(2):274-278. [10]．Dubost N J, Beelman R B, Perterson D, et al.. Identification and Quantification of Ergothioneine in Cultivated Mushrooms by Liquid Chromatography-Mass Spectroscopy[J]. Internationnal Journal of Medicinal Mushroons, 2005,8(8):215-222. [11]．Encarnacion A B, Fagutao F, Hirayama J, et al.. Edible Mushroom (Flammulina velutipes) Extract Inhibits Melanosis in Kuruma Shrimp (Marsupenaeus japonicus)[J]. Journal of Food Science,2011,76(1):C52-C58. [12]．Encarnacion A B, Fagutao F, Hirono I, et al.. Effects of Ergothioneine from Mushrooms (Flammulina velutipes) on Melanosis and Lipid Oxidation of Kuruma Shrimp (Marsupenaeus japonicus)[J]. Journal of Agricultural and Food Chemistry, 2010,58(4):2577-2585. [13]．Ey J, Schouml;mig E, Taubert D. Dietary Sources and Antioxidant Effects of Ergothioneine[J]. Journal of Agricultural and Food Chemistry, 2007,55(16):6466-6474. [14]．Gr#252;ndemann D. The ergothioneine transporter controls and indicates ergothioneine activity -A review[J]. Preventive Medicine, 2012,54:S71-S74. [15]．Lee W Y, Park E, Ahn J K. Supplementation of Methionine Enhanced the Ergothioneine Accumulation in the Ganoderma neo-japonicum Mycelia[J]. Applied Biochemistry and Biotechnology, 2009,158(1):213-221.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备发酵实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写