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毕业论文网 > 任务书 > 化学化工与生命科学类 > 生物工程 > 正文

利用鼠李糖脂一步合成核壳型银颗粒及其抗菌性能的研究任务书

 2020-06-28 20:22:05  

1. 毕业设计(论文)的内容和要求

1.文献查阅 掌握文献查阅,使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源,查阅关于大肠杆菌理性代谢改造方面的相关文献。

2.文献阅读及综述 阅读与课题相关的外文与中文文献,了解国内外的研究动态,撰写文献综述。

3.明确实验任务,拟定实验方案 根据所查阅文献的内容,确定实验内容及方案,拟定科学可行的研究计划。

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2. 参考文献

[1] Syldatk, C., Wagner, F., 1987. Production of biosurfactants. Biosurfactants and Biotechnology 25, 89-120 [2] BANAT I M,FRANZETTI A,UANDOLFI I,et al. Microbial biosurfactants production,applications and future potential.. Appl Microbiol Biotechno1,2010,87(2):427-444. [3] MULLER M M,KUULER J H,HENKEI. M,et al. Rhamnolipid-Next generation surfactants.Biotechnol, 2012,162(4):366-380. [4] Jarvis, F.G., Johnson, M.j., 1949. A glyco-lipide produced by Pseudomonas aeruginosa journal of the American Chemical Society 71, 4124-4126 [5] Syldatk, C., Lang, S., Matulovic, U., Wagner, F., 1985a. Production of four interfacial active rhamnolipids from n-alkanes or glycerol by resting cells of Pseudomonas species DSM 2874. Zeitschrift fur Naturforschung Section C: Biosciences 40,61-67 [6] Andra, J., Rademann, J., Howe, J., Koch, M., Heine, H., Zahringer, U., Brandenburg, K.,2006. Endotoxin-like properties of a rhamnolipid exotoxin from Burkholderia(Pseudomonas) plantarii: immune cell stimulation and biophysical characterization. Biological Chemistry 387, 307-370 [7] Dubeau, D., Deziel, E., Woods, D.E., Lepine, F., 2009. Burkholderia thailandensis harbors two identical rhl gene clusters responsible for the biosynthesis of rhamnolipids. BMC Microbiology 9, 263 [8] Abdel-Mawgoud, A.M., Lepine, F., Deziel, E., 2010. Rhamnolipids: diversity of structures, microbial origins and roles. Applied Microbiology and Biotechnology 86,7323-7336 [9] Muller, M.M., Hausmann, R., 2011. Regulatory and metabolic network of rhamnolipid biosynthesis: traditional and advanced engineering towards biotechnological production. Applied Microbiology and Biotechnology 91,251-264 [10] 程央红.纳米银医学的研发热点[J] . 中国执业药师, 2005,3 ( 15): 33. [11] 程定超,杨洁,赵艳丽.纳米银抗菌材料在医疗器械与生活用品中的应用[J] .医疗卫生装备, 2004, 11: 27-30. [12] Jensen EL, Rungby J, Hansen JC, et al. Serum concentration and accumulation of silver in skin during three months treatment with an anti-smoking chewing gum containing silver acetate [J] .Hum Toxicol, 1988, 7: 535-540. [13] Furno F, Morley KS , Wong B, et al.Silver nanoparticles and polymeric medical devices: a new approach to prevention of infection [J] . J Antimicrob Chemother, 2004, 54( 6): 1019-1024. [14] 郝和平.医疗器械生物学评价标准实施指南[M] .北京: 中 国标准出版社, 2002.100-110. [15] Alt V, Bechert T, Steinrucke P, et al.An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement[J] . Biomaterials, 2004,25( 18): 4383-4391. [16] 梁卫东,石应康.细胞培养法评价生物材料生物相容性研究 进展[J] .生物医学工程学杂志, 1999, 16( 1): 88-92. [17] Mosmann T.Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays [J] . J Immunol Methods.1983, 65( 1-2): 55-63. [18] Hardman R. A toxicologic review of quantum dots: toxicity depends on physicochemical and environmental factors [J] .Environ Health Perspect, 2006, 114( 2): 165-172. [19] Hussain SM, Hess KL, Gearhart JM, et al.In vitro toxicity of nanoparticles in BRL 3A rat liver cells[ J] . Toxicol in vitro , 2005, 19( 7): 975-983.

3. 毕业设计(论文)进程安排

起讫日期 设计(论文)各阶段工作内容 备 注 2017.12-2018.02 文献查阅,完成开题报告,熟悉实验仪器 2018.03-2018.04 优化鼠李糖脂制备银颗粒的反应条件 2018.04-2018.05 产生机理和抗菌性能实验 2018.05-2018.06 补充验证实验,整理实验数据, 撰写毕业论文

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