1. 毕业设计（论文）的内容和要求

构建基于核酸适配体电化学方法制备u型核酸纳米探针及其电化学性能调控，以用于对大肠杆菌、肠毒素进行同时检测分析。

优化组装条件，实现u型核酸纳米探针在金电极表面的快速稳定组装。

u型核酸纳米结构探针相比于简单混合的常规结构探针电化学稳定性明显提升。

2. 参考文献

[1] 用于食品安全检测的生物传感器的研究进展[J]. 蒋雪松,王剑平,应义斌,李延斌. 农业工程学报. 2007(05). [2] Potent inhibition of human immunodeficiency virus type 1 replication by template analog reverse transcriptase inhibitors derived by SELEX (systematic evolution of ligands by exponential enrichment). Joshi Pheroze,Prasad Vinayaka R. Journal of Virology . 2002. [3] Comparing human pancreatic cell secretomes by in vitro aptamer selection identifies cyclophilin B as a candidate pancreatic cancer biomarker [J] . Ray, Partha,Rialon-Guevara, Kristy L,Veras, Emanuela, Sullenger, Bruce A, White, Rebekah R. EN. 2012 (5). [4] Capillary Electrophoresis-SELEX Selection of Aptamers with Affinity for HIV-1 Reverse Transcriptase. Renee K. Mosing, Shaun D. Mendonsa, Michael T. Bowser. Analytical Biochemistry. 2005. [5] Fast determination of the tetracyclines in milk samples by the aptamer biosensor. Zhang Juankun, Zhang Binbin, Wu Yan, Jia Shiru, Fan Ting, Zhang Ziyi, Zhang Chaozheng. Analyst. 2010.

3. 毕业设计（论文）进程安排

3月份完成原理验证 4月份完成条件优化实验、稳定性、重现性实验 5月份完成论文撰写