1. 毕业设计（论文）的内容和要求

我们选择使用生物酶法定向合成所需的稀有人参皂苷。

依靠已有的糖基转移酶，测试其对人参皂苷的催化功能，并找出人参皂苷的催化位点与糖的连接情况。

进一步的实验中，一方面，对酶进行定点改造，优化催化功能和位点选择性；另一方面，测试催化的最适条件，摸索出大批次催化的途径。

2. 参考文献

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3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.12---期末 文献查阅，完成开题报告，熟悉仪器 2018.3---2018.4 了解实验用酶的基本功能、产物的检测及鉴定 2018.4---2018.6 对酶进行定点改造，提高催化能力和位点选择性 2018.5---2018.6 优化反应的条件 2018.6.2---2018.6.10 撰写毕业论文