1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于寡糖分离纯化及表征的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] Kang CZ, Zhou T, Jiang WK, Guo LP, Zhang XB, Xiao CH, Zhao D. Quality evaluation and growing regionalization of Pseudostellaria heterophylla in Guizhou. Zhongguo Zhongyao Zazhi. 2016; 41: 2391-2396. [2] Kang CZ, Zhou T, Jiang WK, Guo LP, Zhang XB, Xiao CH, Zhao D. Research on quality regionalization of cultivated Pseudostellaria heterophylla based on climate factors. Zhongguo Zhongyao Zazhi. 2016; 41: 2386-2390. [3] Wang J, Li J, Li H, Wu X, Gao W. HPLC#8211;ESI#8211;MSn Analysis, Fed-Batch Cultivation Enhances Bioactive Compound Biosynthesis and Immune-Regulative Effect of Adventitious Roots in Pseudostellaria heterophylla. Appl. Biochem. Biotechnol. 2015; 177: 63-75. [4] Zheng Z, Hu J, He Z. A split G-quadruplex and graphene oxide-based low-background platform for fluorescence authentication of Pseudostellaria heterophylla. Sensors. 2014; 14: 22971-22981. [5] Yi J, Liao FP, Zheng WW. Identification of Pseudostellaria heterophylla from different idioplasms by analysis of rDNA ITS sequences. Chin. Trad. Herbal Drugs. 2013; 44: 1318-1322. [6] Wang Z, Liao SG, He Y, Li J, Zhong RF, He X, Liu Y, Xiao TT, Lan YY, Long QD, Wang YL. Protective effects of fractions from Pseudostellaria heterophylla against cobalt chloride-induced hypoxic injury in H9c2 cell. J. Ethnopharmacol. 2013; 147: 540-545. [7] Liu XY, Zhang XX, Wu Y, Lin FY, Cao GZ. Effects of Pseudostellaria heterophylla (Miq.) Pax on the activities of CYP450 enzymes in rats using a cocktail approach. Lat. Am. J. Pharm. 2013; 32: 931-935. [8] Pang W, Lin S, Dai Q, Zhang H, Hu J. Antitussive activity of pseudostellaria heterophylla (Miq.) pax extracts and improvement in lung function via adjustment of multi-cytokine levels. Molecules. 2011; 16: 3360-3370. [9] [Chen FF, Zhang G, Wang YY. Analysis of active constituents in autotetraploid of Pseudostellaria Heterophylla(Miq.). Pharm. Biotechnol. 2010; 17: 523-526. [10] Shen Y, Han C, Liu J, Liu A, Ji X, Liu C. Analysis of volatile components of Pseudostellaria heterophylla (Miq.) Pax by microwave-assisted solvent extraction and GC-MS. Chromatographia. 2008; 68: 679-682. [11] Peng HS, Liu WZ, Hu ZH. Advances in studies on biology and chemical constituents in Radix Pseudostellariae. Chin. Trad. Herbal Drugs. 2008; 39: 470-473. [12] Li Y, Yang XW. Studies on chemical constituents of root tuber of cultivated Pseudostellaria heterophylla (Zheshen No. 1). Zhongguo Zhongyao Zazhi. 2008; 33: 2353-2355. [13] Han C, Shen Y, Chen J, Lee FSC, Wang X. HPLC fingerprinting and LC-TOF-MS analysis of the extract of Pseudostellaria heterophylla (Miq.) Pax root. J. Chromatogr. B Anal. Technol. Biomed. Life Sci. 2008; 862: 125-131. [14] Zhang J, Li YB, Wang DW, Yin ZQ, Duan JA. Chemical constituents from roots Pseudostellaria heterophylla. Zhongguo Zhongyao Zazhi. 2007; 32: 1051-1053. [15] Shen Y, Han C, Chen J, Wang X. Analysis of cyclic peptides in Pseudostellaria heterophylla (Miq.) Pax by HPLC-APCI-MS. Chromatographia. 2007; 66: 319-323. [16] Liu XH, Wang M, Cai BC, Wang YX, Lin XY. GC-MS fingerprint of root tuber of Pseudostellaria heterophylla. Chin. Trad. Herbal Drugs. 2007; 38: 113-116. [17] Li SN, Gao SL, Jiang T. HPLC fingerprints of crude drug of Pseudostellaria heterophylla and root tuber of its autotetraploid plant. J. Plant Resour. Environ. 2007; 16: 36-39. [18] Han C, Chen J, Liu J, Lee FSC, Wang X. Isolation and purification of Pseudostellarin B (cyclic peptide) from Pseudostellaria heterophylla (Miq.) Pax by high-speed counter-current chromatography. Talanta. 2007; 71: 801-805. [19] Han C, Chen J, Chen B, Lee FSC, Wang X. Fingerprint chromatogram analysis of Pseudostellaria heterophylla (Miq.) Pax root by high performance liquid chromatography. J. Sep. Sci. 2006; 29: 2197-2202.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.4-2017.1.13 文献查阅，研究方案制定和开题报告写作 2017.1.14-2017.1.24 开题报告的修改与复核 2017.2.4-2017.2.20 实践内容的准备 2017.2.21-2017.5.10 实践 2017.5.11-2017.5.15 实践内容处理与结果分析 2017.5.16-2017.6.1 毕业论文书写