1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅，使用网络资源如中国期刊网、维普数据库、超星数字图书馆、elsevier电子期刊、springer link全文电子期刊等检索资源，查阅关于脂肪酶产生菌产酶条件优化方面的相关文献。

2．文献阅读及综述 阅读与课题相关的外文与中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] L. Zhu, F. Zhu, and S. Qin, et al, Highly efficient resolution of N -hydroxymethyl vince lactam by solvent stable lipase YCJ01, Journal of Molecular Catalysis B: Enzymatic, 2016, (133):150-156. [2] B. Wang, W.H. Li, B. Wu, B.F. He, Efficient resolution of 3-phenoxy-1,2-propanediol by immobilized lipase on amphiphilic comb polymer modified TiO2, J Mol Catal B-Enzym, 2014, (106):105-110. [3] Baharum S N, Salleh A B, and Razak C N A, et al. Organic solvent tolerant lipase by Pseudomonas sp. strain S5: stability of enzyme in organic solvent and physical factors affecting its production [J]. Ann Microbiol, 2003, (53): 75-83. [4] De Bernardez Clark E, Hevehan D, Szela S, et al. Oxidative renaturation of hen egg-white lysozyme. Folding vs Aggregation. Biotechnol Prog, 1998, (14): 47-54 [5] C.J. Yao, Y. Cao, and B.F. He, et al. An organic solvent and thermally stable lipase from Burkholderia ambifaria YCJ01: purification characteristics and application for chiral resolution of mandelic acid, J. Mol. Catal. B: Enzym. 2012, (85-86):105-110. [6] Timasheff SN: The Control of Protein Stability and Association by Weak-Interactions with Water-How Do Solvents Affect These Processes. Annual Review of Biophysics and Biomolecular Structure, 1993, (22):67-97. [7] Yancey PH, Clark ME, and Hand SC, et al. Somero GN: Living with Water-Stress Evolution of Osmolyte Systems. Science 1982, (217):1214-1222. [8] Emi靗io V. Lage, Sadafara A. Pillai, Haridas Pal, Anita Bahadur, Matilde Casas, Isabel Sa靚dez-Macho, Pratap Bahadur. Urea induced changes in self-assembly and aggregate microstructures of amphiphilic star block copolymers with widely different hydrophobicity. Colloids and Surfaces A 537 (2018) 259?67. [9] Yancey PH, Clark ME, and Hand SC, et al. Living with water stress: evolution of osmolyte systems. Science, 1982, (217):1214-1222. [10] Courtenay ES, Capp MW, and Anderson CF, et al. Vaporpressure osmometry studies of osmolyte-protein interactions: implications for the action of osmoprotectants in vivo and for the inter- pretation of 搊smotic stress?experiments in vitro. Biochemistry, 2000, (39):4455-4471. [11] Bolen DW, Rose G. Structure and energetics of the hydrogen-bonded backbone in protein folding. Annu. Rev. Biochem, 2008, (77) :339-362. [12] Kresheck, G. C.; Scheraga, H. A. The Temperature Dependence of the Enthalpy of Formation of the Amide Hydrogen Bond; the Urea Model, J. Phys. Chem. 1965, (69):1704. [13] T. Artham, N. Mohanalakshmi, P. P. Paragi-Vedanthi, et al. Mechanistic investigations of lipase-catalyzed degradation of polycarbonate in organic solvents[J]. Enzyme and Microbial Technology, 2011, (48): 71-79

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2019.1-2019.2 文献查阅，完成开题报告，熟悉实验仪器。

2019.3-2019.4 融合标签mbp、nusa、gst融合脂肪酶蛋白表达载体的构建。

2019.4-2019.5 高效表达条件的优化以及纯化条件的摸索 2019.5-2019.6 脂肪酶ycj01体外复性条件的探究 2019.6上旬 完成论文及答辩