1. 毕业设计（论文）的内容和要求

1.文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于多糖的分离纯化及结构表征的相关文献，具备文献检索的能力。

2.文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，并撰写综述，提高查找阅读的能力。

3.实验研究 在充分了解当前课题进展的前提下，根据现有条件初步拟定试验方案，确定切实可行的实验计划，并根据实验情况适时调整试验方案。

2. 参考文献

［1］申利红, 王建森, 李雅, 等. 植物多糖的研究及应用进展[J]. 中国农学通报, 2011, 27(2): 349-352 ［2］Deng J，Zhou Y J，Bai M M，et al. Anxiolytic and sedative activities of Passiflora edulis f flavicarpa ［J］. Journal of Ethnopharmacology，2010，128(1) :148－153. ［3］Dhawan K，Kumar S，Sharma A. Anti－anxiety studies on extracts of Passiflora incarnata Linneaus［J］. Journal of Ethnopharmacology，2001，78(2) :165－170. ［4］Juliana V，Zapata Z. Enzymatic extraction of pectin from passion fruit peel( Passilflora edulis f.flavicarpa) at laboratory and bench scale［J］. LWT－Food Science and Technology，2017，80 (3) :280－285. ［5］Contrerasesquivel JC，Aguilar CN，Montanez JC.Pectin from passion fruit fiber and its modification by pectinmethylesterase［J］. Journal of Food Science and Nutrition，2010，15(1) :57－66. ［6］Hongyan Li，Yingxing Wang，Chao Wang，Shaohan Zhang，Shenghui Li，Guoqiang Zhou，Shuxiang Wang，Jinchao Zhang． Extraction，selenylation modification and antitumor activityof the glucan from Castanea mollissima Blume［J］．Glycoconj J，2017，34:207－217． ［7］Shabeena Yousuf Naqash，Ｒ． A． Nazeer． Anticoagulant，Antiherpetic and Antibacterial Activities of Sulphated Polysaccharide from Indian Medicinal Plant Tridax procumbens L．( Asteraceae)［J］．Appl Biochem Biotechnol，2011，165: 902－912． ［8］Luisa Maurelli，Elena Ionata，Francesco La Cara，etc． Chestnut Shell as Unexploited Source of Fermentable． Sugars: Effect of Different Pretreatment Methods on Enzymatic Saccharification［J］．Applied Biochemistry． Biotechnology，2013:170(5) :1104－1118 ［9］王珊，黄胜阳．植物多糖提取液脱蛋白方法的研究进展［J］． 食品科技，2012，37( 9) :188－191． ［10］Sheng－Jun Wu，Lin Yu． Preparation and characterisation of the oligosaccharides derived from Chinese water． chestnut polysaccharides［J］．Food Chemistry，2015，(181) :15－18． ［11］Hongyan Li，Yingxing Wang，Chao Wang，Shaohan Zhang，Shenghui Li，Guoqiang Zhou，Shuxiang Wang，Jinchao Zhang． Extraction，selenylation modification and antitumor activityof the glucan from Castanea mollissima Blume［J］． Glycoconj J，2017，34:207－217． ［12］Christoph Schmitz，Ｒalf Sodian，Use of a plant － based polysaccharide hemostat for the treatment of sternal bleeding after median sternotomy［J］． Schmitz and Sodian Journal of Cardiothoracic Surgery，2015． ［13］Luisa Maurelli，Elena Ionata，Francesco La Cara，etc． Chestnut Shell as Unexploited Source of Fermentable． Sugars: Effect of Different Pretreatment Methods on Enzymatic Saccharification［J］． Applied Biochemistry ． Biotechnology，2013:170( 5) :1104 － 1118 ［14］MingliangJin，QingshengHuang，KeZhao，et al． Biological activities andpotential health benefit effects of polysaccharides isolated from LyciumbarbarumL［J］． International Journal of Biological Macromolecules，2013:16－23． ［15］ZhangZS，WangXM，HanZP，et al． Purification，antioxidant and moisture preserving activities of polysaccharides frompapaya［J］．CarbohydratePolymers，2012，87(3) :2332－2337． ［16］PereiraLdeP，daSilvaＲO，Bringel PH，et al． Polysaccha－ridefractions esalpiniaferreapods:Potential anti －in－flammatoryusage［J］．Journal of Ethnopharmacology，2012，139(2) :642－648． ［17］ZHANG Yangyang, LI Sheng, WANG Xiaohua, et al. Advances in lentinan: isolation, structure, chain conformation and bioactivities[J]. Food ocolloids,2011,25(2):196-206.DOI:10.1016/j.foodhyd.2010.02.001. ［18 PANDA B C, MONDAL S, DEVI S P, et al. Pectic Polysaccharide from the green fruits of Momordica charantia(Karela): structural characterization and study of immunoenhancing and antioxidant properties[J]. Carbohydrate Research, 2015, 401(4): 24-31. DOI:10.1016/j.carres.2014.10.015. ［19］XU Renjie, YE Hong, SUN Yi, et al. Preparation, preliminary characterization, antioxidant, hepatoprotective and antitumor activities of polysaccharides from the flower of tea plant (Camellia sinensis)[J]. Food and Chemical Toxicology, 2012, 50(7): 2473-2480. DOI:10.1016/j.fct.2011.10.047. ［20］LI Jinwei, FAN Liuping, DING Shaodong. Isolation, purification and structure of a new water-soluble polysaccharide fromZizyphus jujubacv. JinSiXiaoZao[J]. Carbohydrate Polymers, 2011, 83(2): 477-482. DOI:10.1016/j.carbpol.2010.08.014.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2019.1.4-2019.1.13 文献查阅，研究方案制定和开题报告写作 2019.1.14-2019.1.24 开题报告的修改与复核 2019.2.4-2019.2.20 实践内容的准备 2019.2.21-2019.5.1 实践 2019.5.11-2019.5.15 实践内容处理与结果分析 2019.5.16-2019.6.1 毕业论文书写