论文总字数：18496字

摘 要

随着科技发展，纳米技术的产业化进程不断加速，许多纳米材料已经大规模生产并应用于涂料、化妆品、催化剂、新型材料、医学等领域的近千种消费产品中。由于小尺寸效应、量子效应和巨大比表面积等因素，纳米材料具有特殊的物理化学性质，在进入生命体后，它们的化学特性和生物活性与化学成分相同的常规物质有很大不同，影响难以评测。纳米材料生物学效应，成为生物医学工程一个重要研究方向。

目前，对纳米材料与生物相互作用的研究，由于其灵敏性以及接近本质的特点，受到广泛的关注，各种分子生物学的研究手段已逐步渗入这一领域。近年来，随着microRNA研究的进一步发展和基于新一代测序技术的高通量表达谱分析手段的引入，我们已能从更深层次，对纳米材料生物学效应进行系统的生物学分析。

本文主要研究金纳米颗粒致miRNA功能表达的差异分析。金纳米颗粒与细胞相互作用中KEGG途径受到一定程度的调控，然而并不是所有基因组都表现显著，只有少部分调控达到了显著的水平。在显著调控的KEGG途径中，细胞信号通路较多的受到金纳米颗粒的影响，体现了金纳米颗粒致miRNA的差异表达是细胞对外界刺激的主动反应。这些KEGG途径不但为已报道的金纳米颗粒作用提供分子水平的诠释，而且还提示可能存在的未经报道的细胞作用。

关键词：金纳米颗粒，microRNA，miRNA表达谱 , KEGG途径

Gold nanoparticles induced differential expression of miRNA functional analysis

Abstract

With technological development , the industrialization process of nanotechnology continues to accelerate , many nanomaterials have been used in large-scale production and coatings, cosmetics , catalysts, new materials , medicine and other fields of nearly a thousand kinds of consumer products.Due to the small size effect, quantum effects and huge surface area and other factors , nano- materials with special physical and chemical properties , after entering the living body , their chemical properties and biological activity of the same chemical composition is very different from conventional materials , the impact is difficult to evaluation. Biological effects of nano-materials, bio- medical engineering to become an important research direction .

Currently , the study of the interaction of nanomaterials with biological , due to its sensitive nature and characteristics close to nature , to receive widespread attention , a variety of research tools in molecular biology has gradually infiltrated this area. In recent years , with further research and development of microRNA -based next-generation sequencing technology, the introduction of high-throughput expression profiling analysis tools , we have from a deeper level , the biological effects of nanomaterials on biological systems analysis .

This paper studies the difference between gold nanoparticles induced the expression of miRNA functional analysis . Gold nanoparticles interact with cells in the KEGG pathway regulating certain extent , but not all have shown significant genome , only a small part of the regulation reached a significant level. In a significant KEGG pathway regulating cell signaling pathways affected more gold nanoparticles , gold nanoparticles reflects differences in miRNA expression is induced by active cellular response to external stimuli . KEGG pathway provides not only the interpretation of these molecular effects of gold nanoparticles have been reported , but there may also be prompted cell function without a coverage.

Key Words: Gold Nanoparticles; microRNA; miRNA expression profiling; KEGG pathway

目录

摘 要 I

Abstract II

第一章 概述 1

1.1 金纳米颗粒的应用 1

1.1.1 纳米材料 1

1.1.2 金纳米颗粒 1

1.1.3 金纳米颗粒的生物安全性 1

1.2 microRNA研究背景及意义 2

1.2.1 microRNA简介 2

1.2.2 miRNA生物合成及功能概述 2

1.2.3 miRNA检测方法 3

1.3 miRNA功能分析的方法 4

1.3.1 miRNA的研究方法 4

1.3.2 mirPath分析软件的介绍 5

1.4 SOLiD高通量测序 6

1.5本论文的研究内容及目标 7

第二章 材料和方法 8

2.1 细胞培养 8

2.2 RNA提取 9

2.3 SOLiD测序 9

2.4 mirPath软件 9

第三章 结果与讨论 11

3.1纳米金颗粒调控NIH/3T3细胞的miRNA表达 11

3.2金纳米颗粒暴露抑制miRNA表达对KEGG途径的调控 12

3.3金纳米颗粒刺激醋精miRNA表达对KEGG途径的调控 15

第四章 结论与展望 17

参考文献 19

致 谢 21

附录 21

第一章 概述

1.1 金纳米颗粒的应用

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