1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于遗传转化体系构建的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]. R Herbst, K Gast and R Seckler. Folding of firefly (Photinus Pyralis) luciferase: aggregation and reactivation of unfolding intermediates[J]. Biochemistry, 1998; 37:6586-6597. [2]. T Masuda, H Tatsumi and E Nakano. Cloning and sequence-analysis of CDNA for luciferase of a japanese firefly, luciola-cruciata[J]. Gene. 1989; 77(77):265-270. [3]. D Frishman, and P Argos.. Knowledge-based protein secondary structure assignment[J]. Proteins. 1995; 23(4):566-579. [4]. 张菊梅，吴清平，周小燕等. 荧光素酶研究进展[J]. 微生物学通报, 2001; 28(5):98-101. [5]. J Brogan, F Li, W Li et al. Imaging molecular pathways: reporter genes[J]. Radiation research. 2012; 177(4):508-513. [6]. H K Kim, E J Cho, S M Jo et al. A split luciferase complementation assay for studying in vivo protein-protein interactions in filamentous ascomycetes[J]. Curr Genet. 2012; 58(3):179-189. [7]. Y Yamakawa, H Ueda, A Kitayama et al. Rapid,homogeneous immunoassay of peptides based on bioluminescence resonance energy transfer from firefly luciferase [J]. J Biosci Bioeng. 2002; 93(6):537-642. [30]. 张琳. 环化蛋白的热稳定性研究[D].上海: 上海交通大学, 2011. [8]. L Li, J O Fierer, T A Rapoport et al. Structural analysis and optimization of the covalent association between SpyCatcher and a peptide tag[J]. J Mol Biol. 2014; 426(2):309-317. [9]. B Zakeria, J O Fierera, E Celikb et al. Peptide tag forming a rapid covalent bond to a protein, through engineering a bacterial adhesin[J]. PNAS. 2012; 109(12):E690#8211;E697. [10]. W B Zhang, F Sun, D A Tirrell et al. Controlling macromolecular topology with genetically encoded SpyTag-SpyCatcher chemistry[J]. J Am Chem Soc. 2013; 135(37) : 13988-13997. [11]. C Schoene, J O Fierer, S P Bennett et al. SpyTag/SpyCatcher cyclization confers resilience to boiling on a mesophilic enzyme[J]. Angew Chem Int Ed. 2014; 53:6101-6104. [12]. J D Wang, Y L Wang, X Z Wang et al. Enhanced thermal stability of lichenase from Bacillus subtilis 168 by SpyTag/SpyCatcher-mediated spontaneous cyclization [J]. Biotechnol Biofuels. 2016; 9:79.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2016.1.2-2016.1.19 文献查询和资料阅读 2016.1.19-2016.1.23 研究方案制定和开题报告写作 2016.1.24-2016.3.10 实践内容的准备 2016.3.11-2016.5.10 实践 2016.5.10-2016.5.15 实践内容处理与结果分析 2016.5.16-2016.6.1 毕业论文书写