1. 毕业设计（论文）的内容和要求

1．文献查阅 掌握文献查阅的一般方法，学会使用计算机在中国期刊网，维普数据库，超星数字图书馆，elsevier、ncbi电子期刊，springer link全文电子期刊，pubmed等检索资源上查阅关于寡糖分离纯化及表征的相关文献。

2．文献阅读及综述 阅读与课题相关的外文及中文文献，了解国内外的研究动态，撰写文献综述。

3．明确实验任务，拟定实验方案 根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1] J. Li, K.L. Cheong, J. Zhao, D.J. Hu, X.Q. Chen, C.F. Qiao, Q.W. Zhang, Y.W. Chen, S.P. Li, Preparation of inulin-type fructooligosaccharides using fast protein liquid chromatography coupled with refractive index detection, Journal of Chromatography A 1308 (2013) 52-57. [2] W.R. Zong, K.L. Cheong, D.T. Wu, J. Li, J. Zhao, S.P. Li, Preparation and purification of raffinose family oligosaccharides from Rehmannia glutinosa Libosch. by fast protein liquid chromatography coupled with refractive index detection, Separation and Purification Technology 138 (2014) 98-103. [3] V. Bali, P.S. Panesar, M.B. Bera, R. Panesar, Fructo-oligosaccharides: Production, Purification and Potential Applications, Critical Reviews in Food Science and Nutrition 55 (2015) 1475-1490. [4] B. G#243;mez, B. Gull#243;n, C. Remoroza, H.A. Schols, J.C. Paraj#243;, J.L. Alonso, Purification, characterization, and prebiotic properties of pectic oligosaccharides from orange peel wastes, Journal of Agricultural and Food Chemistry 62 (2014) 9769-9782. [5] T. Liang, Q. Fu, F. Li, W. Zhou, H. Xin, H. Wang, Y. Jin, X. Liang, Hydrophilic interaction liquid chromatography for the separation, purification, and quantification of raffinose family oligosaccharides from Lycopus lucidus Turcz, Journal of Separation Science 38 (2015) 2607-2613. [6] D.J. Hu, K.L. Cheong, J. Zhao, S.P. Li, Chromatography in characterization of polysaccharides from medicinal plants and fungi, Journal of Separation Science 36 (2013) 1-19. [7] J. Li, X.M. Liu, B. Zhou, J. Zhao, S.P. Li, Determination of Fructooligosaccharides in Burdock Using HPLC and Microwave-Assisted Extraction, Journal of Agricultural and Food Chemistry 61 (2013) 5888-5892. [8] S.P. Li, D.T. Wu, G.P. Lv, J. Zhao, Carbohydrates analysis in herbal glycomics, TrAC - Trends in Analytical Chemistry 52 (2013) 155-169. [9] R. Montella, J.D. Co#239;sson, F. Travaglia, M. Locatelli, M. Bordiga, M. Meyrand, D. Barile, M. Arlorio, Identification and characterisation of water and alkali soluble oligosaccharides from hazelnut skin (Corylus avellana L.), Food Chemistry 140 (2013) 717-725. [10] L.R. Ruhaak, A.M. Deelder, M. Wuhrer, Oligosaccharide analysis by graphitized carbon liquid chromatography-mass spectrometry, Analytical and Bioanalytical Chemistry 394 (2009) 163-174. [11] D.W. Templeton, M. Quinn, S. Van Wychen, D. Hyman, L.M.L. Laurens, Separation and quantification of microalgal carbohydrates, Journal of Chromatography A 1270 (2012) 225-234. [12] K. Yamada, K. Kakehi, Recent advances in the analysis of carbohydrates for biomedical use, Journal of Pharmaceutical and Biomedical Analysis 55 (2011) 702-727. [13] X. Zhao, B. Yang, L. Li, F. Zhang, R.J. Linhardt, On-line separation and characterization of hyaluronan oligosaccharides derived from radical depolymerization, Carbohydrate Polymers 96 (2013) 503-509.

3. 毕业设计（论文）进程安排

2016.1.4-2016.1.15 文献查阅，研究方案制定和开题报告写作 2016.1.16-2016.2.7 开题报告的修改与复核 2016.2.17-2016.3.1 实践内容的准备 2016.3.2-2016.5.10 实践 2016.5.11-2016.5.15 实践内容处理与结果分析 2016.5.16-2016.6.1 毕业论文书写