1. 毕业设计（论文）的内容和要求

要求毕业论文围绕纤维二糖差向异构酶的定点突变进行阐述，结合实验结果，分析定点突变对酶学性质提升的效果。

争取挖掘出适合工业化的纤维二糖差向异构酶突变体。

2. 参考文献

(1) Shen, Q., Zhang, Y., Yang, R., Hua, X., Zhang, W., Zhao, W. (2015). Thermostability enhancement of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus by site-directed mutagenesis. Journal of Molecular Catalysis B: Enzymatic, 120, 158-164. (2) Park, A. R., Kim, J. S., Jang, S. W., Park, Y. G., Koo, B. S., Lee, H. C. (2017). Rational modification of substrate binding site by structure-based engineering of a cellobiose 2-epimerase in Caldicellulosiruptor saccharolyticus. Microbial cell factories, 16(1), 224. (3) Shen, Q., Zhang, Y., Yang, R., Pan, S., Dong, J., Fan, Y., Han, L. (2016). Enhancement of isomerization activity and lactulose production of cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus. Food chemistry, 207, 60-67.

3. 毕业设计（论文）进程安排

2017.10.10-2017.10.29 确定论文题目、填写任务书 2017.10.30-2017.11.6 毕业设计（论文）任务书及进度表 2017.11.7-2017.12.6 查找资料、写开题报告 2017.12.7-2018.2.12 初步实验及验证 2018.3.1-2018.3.30 完善实验、数据处理 2018.4.1-2018.4.15 论文写作 2018.4.16-2018.4.25 完成论文初稿交老师批阅 2018.4.26-2018.5.15 论文修改 2018.5.16-2018.6.1 论文答辩准备