1. 毕业设计（论文）的内容和要求

本项目拟通过深层发酵银耳菌株的方法高效制备银耳多糖，改进提取纯化工艺，提高胞外多糖产量，解析银耳胞外多糖的分子量、一级结构等特征参数。

2. 参考文献

[1] Ukai S, Kiho T, Hirose K. Isolations and Characterizations of Polysaccharides from Tremella fuciformis BERK[J]. Chemical Pharmaceutical Bulletin, 1972, 20(6):1347-1348. [2] GAO Q P, SELJELID R, CHEN H Q, et al. Characterisation of acidicheteroglycans from Tremella fuciformis Berk with cytokine stimulating activity[J]. Carbohydr Res, 1996, 288: 135-142 [3] GAO Q P, JIANG R Z, CHEN H Q, et al. Characterization and cytokine stimulating activities of heteroglycans from Tremella fuciformis[J].Planta Med, 1996, 62: 297-302. [4] GAO Q P, KILLIE M K, CHEN H C, et al. Characterization and cytokine-stimulating activities of acidic heteroglycans from Tremella fuciformis[J]. Planta Med, 1997, 63: 457-460. [5] 王瑞雪, 颜军, 邬晓勇,等. 液体深层发酵条件对银耳菌丝体酸性多糖富集的影响[J]. 食品科学, 2009, 30(21):175-177. [6] 朱虎, 曹春旭. Method for fermenting to produce tremellan by staged pH value control policy:, CN 102041286 B[P]. 2013.

3. 毕业设计（论文）进程安排

2017.11-2018.01 准备实验所需试剂和设备，完成银耳菌的菌种鉴定、发酵培养条件优化。

2018.03-2018.04 胞外银耳多糖的提取纯化 2018.04-2018.05 纯化后胞外银耳多糖性质分析、结构初步鉴定，完成毕业论文撰写。