1. 毕业设计（论文）的内容和要求

1、采用计算机检索20篇中文文献和5篇英文文献； 2、阅读20篇文献，翻译一篇英文文献，提交一篇开题报告。

格式要求为：参考文献标注符合规范，计算机打印； 3、实验工作主要内容 4、中期报告 以powerpoint制作中期报告，必须对课题的研究背景、文献分析的现及初 步实验结果进行汇报。

5、毕业论文 1）实验方案可行，实验工作量饱满； 2）数据计算准确可靠，文字流畅，图表规范（计算机作图）； 3）分析讨论的证据充分，反映一定的理论基础。

2. 参考文献

[1]Shima S, Sakai H. Poly-L-lysine produced by Streptomyces. Part II. Taxonomy and fermentation studies[J]. Agricultural and Biological Chemistry, 1981, 45(11): 2497-2502. [2]Shima S, MATSUOKA H, IWAMOTO T, et al. Antimicrobial action of. EPSILON.-poly-L-lysine[J]. The Journal of antibiotics, 1984, 37(11): 1449-1455. [3]Vaara M, Vaara T. Polycations sensitize enteric bacteria to antibiotics[J]. Antimicrobial Agents and Chemotherapy, 1983, 24(1): 107-113. [4]Vaara M. Agents that increase the permeability of the outer membrane[J]. Microbiological reviews, 1992, 56(3): 395-411. [5]Delihas N, Riley L W, Loo W, et al. High sensitivity of Mycobacterium species to the bactericidal activity by polylysine[J]. FEMS microbiology letters, 1995, 132(3): 233-237. [6]刘慧, 徐红华. 聚赖氨酸抑菌性能的研究[J]. 东北农业大学学报, 2000, 31(3): 294-298. [7]Koglin A, Walsh C T. Structural insights into nonribosomal peptide enzymatic assembly lines.[J]. Natural Product Reports, 2009, 40(44):987-1000. [8]Dr, Henning D. Mootz, D. S. Dipl.-Chem, and M. A. M. Dr. "Ways of Assembling Complex Natural Products on Modular Nonribosomal Peptide Synthetases." Chembiochem 3.6(2002):490#8211;504. [9]Schneider A, Marahiel M A. Genetic evidence for a role of thioesterase domains, integrated in or associated with peptide synthetases, in non-ribosomal peptide biosynthesis in Bacillus subtilis[J]. Archives of Microbiology, 1998, 169(5):404-410. [10]Strieker M, Tanovi#55030; A, Marahiel M A. Nonribosomal peptide synthetases: structures and dynamics.[J]. Current Opinion in Structural Biology, 2010, 20(2):234. [11]Gr#252;newald J, Marahiel M A. Chemoenzymatic and Template-Directed Synthesis of Bioactive Macrocyclic Peptides[J]. Microbiol Mol Biol Rev, 2006, 70(1):121-146. [12]Mootz H D, Marahiel M A. Biosynthetic systems for nonribosomal peptide antibiotic assembly[J]. Current Opinion in Chemical Biology, 1997, 1(4):543-51. [13]Dr, Henning D. Mootz, D. S. Dipl.-Chem, and M. A. M. Dr. "Ways of Assembling Complex Natural Products on Modular Nonribosomal Peptide Synthetases." Chembiochem 3.6(2002):490#8211;504. [14]Chang K H, Xiang H, Dunaway-Mariano D. Acyl-adenylate motif of the acyl-adenylate/thioester-forming enzyme superfamily: a site-directed mutagenesis study with the Pseudomonas sp. strain CBS3 4-chlorobenzoate:coenzyme A ligase[J]. Biochemistry, 1997, 36(50):15650-9. [15]Gulick A M. Conformational dynamics in the Acyl-CoA synthetases, adenylation domains of non-ribosomal peptide synthetases, and firefly luciferase.[J]. Acs Chemical Biology, 2009, 4(10):811-827. [16]Mcquade T J, Shallop A D, Sheoran A, et al. A nonradioactive high-throughput assay for screening and characterization of adenylation domains for nonribosomal peptide combinatorial biosynthesis[J]. Analytical Biochemistry, 2009, 386(2):244-250.

3. 毕业设计（论文）进程安排

2017.10.10--2017.10.29 确定论文题目、填写任务书 2017.10.30--2017.11.6 毕业设计（论文）任务书及进度表 2017.11.7--2018.1.13 查找资料、写开题报告 2018.1.16--2018.2.15 初步实验及验证 2018.2.16--2018.3.31 完善实验、数据处理 2018.4.1--2018.4.15 论文写作 2018.4.16--2018.4.25 完成论文初稿 2018.4.26--2018.5.15 论文修改 2018.5.16--2018.6.1 论文答辩准备