1. 毕业设计（论文）的内容和要求

内容：通过抑制大肠杆菌NAD(H)合成途径上的关键基因nadD，研究nadD基因转录受限对L-苹果酸合成的影响。

要求：独立设计实验方案及流程；熟练掌握相关实验操作；完成外文翻译，开题报告，毕业论文撰写。

2. 参考文献

[1] Qi L S , Larson M H , Gilbert L A , et al. Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression[J]. Cell, 2013, 152(5):1173-1183. [2] Chi Z , Wang Z P , Wang G Y , et al. Microbial biosynthesis and secretion of l-malic acid and its applications[J]. Critical Reviews in Biotechnology, 2014. [3] Basan M , Hui S , Okano H , et al. Overflow metabolism in Escherichia coli results from efficient proteome allocation.[J]. Nature, 2015, 528(7580):99-104. [4] Knabben I , Regestein L , Marquering F , et al. High cell-density processes in batch mode of a genetically engineered Escherichia coli strain with minimized overflow metabolism using a pressurized bioreactor[J]. Journal of Biotechnology, 2010, 150(1):73-79. [5] Hovejensen B . Phosphoribosyl diphosphate synthetase-independent NAD de novo synthesis in Escherichia coli: a new phenotype of phosphate regulon mutants.[J]. Journal of Bacteriology, 1996, 178(3):714.

3. 毕业设计（论文）进程安排

2018.12.01-2019.01.01 查阅相关文献 2019.01.02-2019.01.08 完成外文翻译 2019.01.09-2019.01.16 完成开题报告 2019.02.16-2019.03.16 nadD基因敲低实验 2019.03.17-2019.05.16 利用重组大肠杆菌合成L-苹果酸实验 2019.05.17- 毕业论文和答辩