基于几丁质结合模块的分子探针构建及其应用研究任务书
2020-05-01 08:41:53
1. 毕业设计(论文)的内容和要求
掌握文献查阅, 使用网络资源如中国期刊网、 维普数据库、 超星数字图书 馆、 elsevier 电子期刊、 springer link 全文电子期刊等检索资源, 查阅关于碳水化合物结合模块方面的相关文献。
2. 文献阅读及综述 阅读与课题相关的外文与中文文献,了解国内外的研究动态, 撰写文献综 述。
3. 明确实验任务, 拟定实验方案 根据所查阅文献的内容, 确定实验内容及方案, 拟定科学可行的研究计划。
2. 参考文献
1 Carbohydrate-active enzymes: an integrated database approach. In Recent Advances in Carbohydrate Bioengineering 2 Advances in molecular engineering of carbohydrate-binding modules 3 Recombinant CBM-fusion technology #8212; Applications overview 4 Recombinant protein expression and purification: A comprehensive review of affinity tags and microbial applications 5 Rodriguez, Beatriz, et al. "Inexpensive and generic affinity purification of recombinant proteins using a family 2a CBM fusion tag." Biotechnology progress 20.5 (2004): 1479-1489 6 Demishtein, Alik, et al. "Characterization of a dockerin‐based affinity tag: application for purification of a broad variety of target proteins." Journal of Molecular Recognition 23.6 (2010): 525-535. 7 One-step purification and immobilization of thermophilic polyphosphate glucokinase from Thermobifida fusca YX: glucose-6-phosphate generation without ATP 8 Molecular characterization of a novel chitinase CmChi1 from Chitinolyticbacter meiyuanensis SYBC-H1 and its use in N-acetyl-d-glucosamine productio
3. 毕业设计(论文)进程安排
2018.12-2019.02 文献查阅, 完成开题报告, 熟悉实验仪器 2019.03-2019.04 异源表达蛋白分子探针 2019.04-2019.05 经行分子探针性能测试,考察其实际应用效果。
2019.05-2019.06 补充验证实验, 整理实验数据,撰写毕业论文