1. 毕业设计（论文）的内容和要求

1,5-戊二胺是l-赖氨酸的衍生物，由于其结构和性质与石油化工来源的己二胺类似，可替代己二胺用于聚酰胺、聚氨酯、重金属结合剂等产品的生产中。

随着石化资源的日渐枯竭，生物法合成戊二胺成为研究的热点。

全细胞催化法合成戊二胺相对于直接发酵法和化学合成法操作相对简单，生产效率高，目标产物浓度高，且成分较为单一，降低了下游分离纯化的难度和成本。

2. 参考文献

[1] Becker J, Wittmann C. Bio-based production of chemicals, materials and fuels -Corynebacterium glutamicum as versatile cell factory [J]. Current opinion in biotechnology, 2012, 23(4): 631-640. [2] Schneider J, Wendisch V F. Biotechnological production of polyamines by bacteria: recent achievements and future perspectives [J]. Applied microbiology and biotechnology, 2011, 91(1): 17-30. [3] Carus M, Scholz L. Biowerkstoff-Report: Report on Bio-based Plastics and Composites [M]. 4th International Congress on Bio-based Plastics and Composites. Maternushaus, Cologne (Germany). 2011. [4] Kind S, Wittmann C. Bio-based production of the platform chemical 1,5-diaminopentane [J]. Applied microbiology and biotechnology, 2011, 91(5): 1287-1296. [5] Zajkoska P, Rebros M, Rosenberg M. Biocatalysis with immobilized Escherichia coli [J].Applied microbiology and biotechnology, 2013, 97(4): 1441-1455. [6] Mateus D M R, Alves S S, Da Fonseca M M R. Diffusion in cell-free and cell immobilising κ-carrageenan gel beads with and without chemical reaction [J]. Biotechnology and Bioengineering, 1999, 63(5): 625-631. [7] Nishi K, Endo S, Mori Y, et al. Method for producing cadaverine dicarboxylate and its use for the production of nylon [P]. EU: EP1482055 (B1), 2006.1.3. [8] Oh Y H, Kang K H, Kwon M J, et al. Development of engineered Escherichia coli whole-cell biocatalysts for high-level conversion of L-lysine into cadaverine [J]. J Ind Microbiol Biotechnol, 2015, 42(11): 1481-1491. [9] Li N, Chou H, Yu L, et al. Cadaverine production by heterologous expression of Klebsiella oxytoca lysine decarboxylase [J]. Biotechnology and Bioprocess Engineering, 2014, 19(6): 965-972. [10] Wang C, Zhang K, Zhongjun C, et al. Directed evolution and mutagenesis of lysine decarboxylase from Hafnia alvei AS1.1009 to improve its activity toward efficient cadaverine production [J]. Biotechnology and Bioprocess Engineering, 2015, 20(3): 439-446. [11] Kim H J, Kim Y H, Shin J H, et al. Optimization of direct lysine decarboxylase biotransformation for cadaverine production with whole-cell biocatalysts at high lysine concentration [J]. Journal of microbiology and biotechnology, 2015, 25(7): 1108-1113. [12] Sabo D L, Fischer E H. Chemical properties of Escherichia coli lysine decarboxylase including a segment of its pyridoxal 5'-phosphate binding site [J]. Biochemistry, 1974, 13(4): 670-676. [13] Bhatia S K, Kim Y H, Kim H J, et al. Biotransformation of lysine into cadaverine using barium alginate-immobilized Escherichia coli overexpressing CadA [J]. Bioprocess and Biosystems Engineering, 2015, 38(12): 2315-2322. [14] 蒋丽丽, 刘均忠, 沈俞, 等. 用固定化 L-赖氨酸脱羧酶细胞制备 1,5-戊二胺 [J]. 精细化工, 2007, 24(1): 1080-1084.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 2018.1.1-1.10 选题，与导师讨论题目 2018.1.11 导师下达任务书 2018.1.12-2018.2.8 根据任务书要求，查找相关文献资料，完成开题报告 2018.02.9-05.23 完成试验并提交论文第一稿 2018.05.24-05.27 根据导师意见修改论文第一稿，完成并提交第二稿 2018.05.28-06.1 根据导师意见修改论文第二稿，完成并提交第三稿 2018.06.1-06.7 完成论文的审阅，准备答辩