1. 毕业设计（论文）的内容和要求

内容 1.探究不同种类的植物激素对裂殖壶菌生长和油脂积累及其组份变化的影响。

2.探究不同浓度植物激素对裂殖壶菌的影响，确定最佳作用浓度。

3.探究在恶劣条件下植物激素对裂殖壶菌生长抑制及油脂变化的缓解作用。

2. 参考文献

[1] Santner A, Calderon-Villalobos L I A, Estelle M. Plant hormones are versatile chemical regulators of plant gr owth[J]. Nat Chem Biol, 2009, 5: 301-307.  [2] 熊国胜，李家洋，王永红. 植物激素调控研究进展[J]. 科学通报, 2009, 54(18):2718-2733 [3] M Jusoh, SH Loh,#8194;TS Chuah, et al.#8194;Elucidating the role of jasmonic acid in oil accumulation, fatty acid composition and gene expression in Chlorella vulgaris(Trebouxiophyceae) during early stationary growth phase[J]. Algal Research, 2015, 9:14-20. [4] T Liu, F Liu, C Wang, et al. The boosted biomass and lipid accumulation in Chlorella vulgaris by supplementation of synthetic phytohormone analogs[J]. Bioresource Technology, 2017, 232:44-52. [5] 郝宗娣, 刘平怀, 时杰, 等.不同植物激素对原始小球藻生长及油脂含量的影响[J]. 广东农业科技，2012, 39（8）：000104-107. [6] J Liu, W Qiu, Y Song. Stimulatory effect of auxins on the growth and lipid productivity of Chlorella pyrenoidosa and Scenedesmus quadricauda[J]. Algal Research, 2016, 18:273-280. [7] XJ Yu, J Sun, YQ Sun, et al. Metabolomics analysis of phytohormone gibberellin improving lipid and DHA accumulation in Aurantiochytrium sp[J]. Biochemical Engineering Journal, 2016, 112:258-268. [8] P Rattanapoltee, W Chulalaksananukul, AE James, et al. Comparison of autotrophic and heterotrophic cultivations of microalgae as a raw material for biodiesel production[J]. Journal of Biotechnology, 2008, 136(4):S412-S412. [9] YD Lu, P Jiang, SF Liu, et al. Methyl jasmonate- or gibberellins A3-induced astaxanthin accumulation is associated with up-regulation of transcription of β-carotene ketolase genes (bkts) in microalga Haematococcus pluvialis[J]. Bioresource Technology, 2010,101(16):6468-6474. [10] V Raman, S Ravi. Effect of salicylic acid and methyl jasmonate on antioxidant systems of Haematococcus pluvialis[J]. Acta Physiologiae Plantarum, 2011, 33(3):1043-1049. [11] 崔宝霞. 雨生红球藻712株生产虾青素研究[D]. 武汉工业学院 ,#160;2008. [12] 吴克刚, 柴向华, 杨连生. 植物激素对破囊壶菌生长与产 DHA 的影响[J]. 微生物学报, 2003, 43(1):111-115. [13] 许智宏, 李家洋. 植物激素对破囊壶菌生长与产 DHA 的影响[J]. 植物学报, 2006, 23(5):433-442. [14] Vierstra R D. The ubiquitin-26S proteasome system at the nexus of plant biology[J]. Nat Rev Mol Cell Biol, 2009, 10: 385#8212;397. [15] X Yu, J Sun, J Zheng, et al. Metabolomics analysis reveals 6-benzylaminopurine as a stimulator for improving lipid and DHA accumulation of Aurantiochytrium sp[J]. Journal of Chemical Technology Biotechnology, 2016, 91(4):1199-1207. [16] SB Sulochana, M Arumugam. Influence of abscisic acid on growth, biomass and lipid yield of Scenedesmus quadricauda under nitrogen starved condition[J]. Bioresource Technology, 2016, 213:198. [17] AG Babu, X Wu, AN Kabra, et al.#8194;Cultivation of an indigenous Chlorella sorokiniana with phytohormones for biomass and lipid production under N-limitation[J]. Algal research, 2017, 23:178-185. [18] K Yoshida, E Igarashi, E Wakatsuki, et al. Mitigation of osmotic and salt stresses by abscisic acid through reduction of stress-derived oxidative damage in Chlamydomonas reinhardtii[J]. Plant Science, 2004, 167(6):1335-1341. [19] 傅华龙, 何天久, 吴巧玉. 植物生长调节剂的研究与应用[J]. 生物加工过程, 2008, 6(4):7-12. #8194;

3. 毕业设计（论文）进程安排

12.30-01.30 查阅文献，完成开题报告 01.30#8212;03.01 完成英文翻译 03.01-05.30 完成实验工作 06.01-06.10 完成毕业论文