1. 毕业设计（论文）的内容和要求

内容： 1、考察不同温度对裂殖壶菌发酵产EPA的影响 2、确定合适的低温条件，通过连续驯化，获得一株高产EPA菌株 3、分析原始菌株与高产菌株的代谢差异，剖析高产菌株的低温调控机理 要求： 1、查阅文献，了解课题背景与研究现状 2、熟悉实验室的基本操作，安全规范使用实验仪器。

3、及时记录并整理实验数据 4、完成开题报告和毕业论文的写作

2. 参考文献

[1] Arner P, Bernard S, Salehpour M, Possnert G, Liebl J, Steier P, Buchholz BA, Eriksson M, Arner E, Hauner H,Skurk T, Ryden M, Frayn KN, Spalding KL. Dynamics of human adipose lipid turnover in health and metabolic disease [J]. Nature 2011, 478: 110-113. [2] Sijtsma L, De Swaaf ME. Biotechnological production and applications of the ω-3 polyunsaturated fatty acid docosahexaenoic acid [J]. Applied Microbiology and Biotechnology, 2004, 64: 146-153 [3] Yazawa K. Production of eicosapentaenoic acid from marine bacteria[J]. Lipids, 1996, 31(1):S297-S300. [4] Barclay W R. Process for the heterotrophic production of microbial products with high concentrations of omega-3 highly unsaturated fatty acids[J]. At Au Bb Bg Br Ca Ch De Dk Es Fi Gb Hu Jp Kp Kr Lk Lu Mc Mg Mw Nl, 1997. [5] Zhao X, Ren L, Guo D, et al. CFD investigation of Schizochytrium [J]. Bioprocess and biosystems engineering, 2016, 39(8): 1297-1304. [6] 马国红, 许鹏, 宋理平,等. 影响微藻EPA含量的环境因素研究进展[J]. 广东海洋大学学报, 2014, 34(6):103-108. [7] Hillig, F.; Porscha, N.; Junne, S.; Neubauer, P. Growth and docosahexaenoic acid production performance of the heterotrophic marine microalgae Crypthecodinium cohnii in the wave-mixed single-use reactor CELL-tainer. Eng. Life Sci. 2014, 14, 254#8722;263. [8] Xie X, Meesapyodsuk D, Qiu X. Functional analysis of the dehydratase domains of a PUFA synthase from Thraustochytrium in Escherichia coli[J]. Applied Microbiology Biotechnology, 2017, 102(7):1-10. [9] Lee S J, Seo P S, Kim C H, et al. Isolation and characterization of the eicosapentaenoic acid biosynthesis gene cluster from Shewanella sp. BR-2[J]. Journal of Microbiology Biotechnology, 2009, 19(9):881. [10] Ren L, Zhou P, Zhu Y, et al. Improved eicosapentaenoic acid production in Pythium splendens RBB-5 based on metabolic regulation analysis.[J]. Appl Microbiol Biotechnol, 2017, 101(9):1-12. [11] L.-J. Ren, H. Huang, A.-H. Xiao, M. Lian, L.-J. Jin, X.-J. Ji, Enhanced docosahexaenoic acid production by reinforcing acetyl-CoA and NADPH supply in Schizochytrium sp. HX-308, Bioprocess Biosyst. Eng. 32 (6) (2009) 837#8211;843. [12] Elrazak A A, Ward A C, Glassey J. Response surface methodology for optimising the culture conditions for eicosapentaenoic acid production by marine bacteria[J]. Journal of Industrial Microbiology Biotechnology, 2013, 40(5):477-487. [13] E. Ryckebosch, K. Muylaert, I. Foubert, Optimization of an analytical procedure for extraction of lipids from microalgae, J. Am. Oil Chem. Soc. 89 (2) (2011) 189#8211;198 [14] Kawamoto J, Kurihara T K, Nagayasu M, et al. Eicosapentaenoic acid plays a beneficial role in membrane organization and cell division of a cold-adapted bacterium, Shewanella livingstonensis Ac10[J]. Journal of Bacteriology, 2009, 191(2):632-40. [15] Ma Z, Tan Y, Cui G, et al. Transcriptome and gene expression analysis of DHA producer Aurantiochytrium under low temperature conditions[J]. Scientific Reports, 2015, 5:14446.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 12.30-01.30 查阅文献，完成开题报告 01.30#8212;03.01 完成英文翻译 03.01-05.30 完成实验工作 06.01-06.10 完成毕业论文