1. 毕业设计（论文）的内容和要求

（1）掌握查阅文献的一般方法，学会在一些网站上查阅相关文献。

需要在中国期刊网、web of science科学引文索引、springer link电子期刊等检索资源上查阅关于酶催化法合成莱鲍迪甙d研究的相关文献，全面了解诱导大肠杆菌表达目的蛋白的原理及酶最适催化条件。

（2）文献阅读及综述：阅读与课题相关的中英文文献，了解国内外的研究动态，撰写文献综述。

2. 参考文献

[1] 刘欢, 李艳, 严明, 等. 重组酿酒酵母全细胞催化合成莱鲍迪苷A[J]. 食品与发酵工业, 2012, 38(7): 6-10. [2] Prakash, I.; Bunders, C.; Devkota, K.P.; Charan, R.D.; Ramirez, C.; Priedemann, C.; Markosyan, A. Isolation and Characterization of a Novel Rebaudioside M Isomer from a Bioconversion Reaction of Rebaudioside A and NMR Comparison Studies of Rebaudioside M Isolated from Stevia rebaudiana Bertoni and Stevia rebaudiana Morita. Biomolecules 2014, 4, 374#8211;389. [3] Prakash, I.; Bunders, C.; Devkota, K.P.; Charan, R.D.; Ramirez, C.; Priedemann, C.; Markosyan, A. Isolation and Structure Elucidation of Rebaudioside D2 from Bioconversion Reaction of Rebaudioside A to Rebaudioside D. Nat. Prod. Commun. 2014, 9, 1135#8211;1138. [4] Mikkelsen, M.D.; Hansen, J.; Simon, E.; Brianza, F.; Semmler, A.; Olsson, K.; Carlsen, S.; During, L.; Ouspenski, A.; Hicks, P. Methods for Improved Production of Redaudioside D and Rebaudioside M. WO2014/122227, 14 August 2014. [5] Wayne, E.S.; Lin, L. NMR studies of the conformation of the natural sweetener rebaudioside A. Carbohydr. Res. 2009, 344, 2533#8211;2538. [6] Prakash, I.; Campbell, M.; Chaturvedula, V.S.P. Catalytic hydrogenation of the sweet principles of Stevia rebaudiana, rebaudioside B, rebaudioside C, and rebaudioside D and sensory evaluation of their reduced derivatives. Int. J. Mol. Sci. 2012, 13, 15126#8211;15136. [7] Clos, J.F.; DuBois, G.E.; Prakash, I. Photostability of rebaudioside A and stevioside in beverages. J. Agric. Food Chem. 2008, 56, 8507#8211;8513. [8] (a) Carakostas MC, Curry LL, Boileau AC, Brusick DL. (2008) Overview: the history, technical function and safety of rebaudioside A, a naturally occurring steviol glycoside, for use in food and beverage. Food and Chemical Toxicity, 46, S1-S10; (b), Chaturvedula VSP, Prakash I. (2012) Spectral analysis and chemical studies of the sweet constituent, rebaudioside A. European Journal of Medicinal Plants, 2, 57-65. [9] 刘欢,李艳,严明, 等.甜叶菊糖基转移酶UGT76G1的克隆表达及其性质研究.食品工业技, 2012, 33(20)：187-190. [10] Vogt T, Jones P. Glycosyltransferases in plant natural product synthesis: characterization of a supergene family[J]. Trends Plant Sci, 2000, 5 (9): 380-386.

3. 毕业设计（论文）进程安排

2017.1-2017.3 熟悉实验原理，掌握实验操作，查阅相关文献，对课题进行了解与探索。

2017.3-2017.4 利用基因工程方法分别构建带有糖基转移酶ugtsl2，ugtd1，ugteugt三株bl21(de3)重组菌株。

2017.4-2017.5 对已构建菌株进行诱导表达，糖基转移酶催化产物检测方法的确定，及选出已构建三株菌株产莱苞迪甙d最优菌株。