1. 毕业设计（论文）的内容和要求

长臂羧酸花菁染料同时具有荧光、色度和易与稀土配位组装，能够拓展的花菁类探针应用于稀土纳米检测方法，通过溴代偶联反应将长臂羧酸烷基链连接上花菁染料上，可以制得长臂羧酸的易组装于上转换纳米晶探针平台。

本实验以溴化长臂羧酸链和吲哚衍生物为原料合成花菁染料探针，并通过1H-NMR、MS等分析方法对其结构进行表征，为合成组装上转换长臂羧酸花菁染料纳米探针做前期合成准备。

2. 参考文献

(1) Park, Y. I.; Lee, K. T.; Suh, Y. D.; Hyeon, T. Upconverting nanoparticles: a versatile platform for wide-field two-photon microscopy and multi-modal in vivo imaging. Chem. Soc. Rev. 2015, 44 (6), 1302-1317. (2) Zhou, Y.; Chen, W.; Zhu, J.; Pei, W.; Wang, C.; Huang, L.; Yao, C.; Yan, Q.; Huang, W.; Loo, J. S. C.; Zhang, Q. Inorganic-Organic Hybrid Nanoprobe for NIR-Excited Imaging of Hydrogen Sulfide in Cell Cultures and Inflammation in a Mouse Model. Small 2014, 10 (23), 4874-4885. (3) Wang, C.; Cheng, L.; Liu, Z. Drug delivery with upconversion nanoparticles for multi-functional targeted cancer cell imaging and therapy. Biomaterials 2011, 32 (4), 1110-1120. (4) Zhou, J.-C.; Yang, Z.-L.; Dong, W.; Tang, R.-J.; Sun, L.-D.; Yan, C.-H. Bioimaging and toxicity assessments of near-infrared upconversion luminescent NaYF4:Yb,Tm nanocrystals. Biomaterials 2011, 32 (34), 9059-9067. (5) Xiong, L.; Yang, T.; Yang, Y.; Xu, C.; Li, F. Long-term in vivo biodistribution imaging and toxicity of polyacrylic acid-coated upconversion nanophosphors. Biomaterials 2010, 31 (27), 7078-7085. (6) Liu, Y.; Chen, M.; Cao, T.; Sun, Y.; Li, C.; Liu, Q.; Yang, T.; Yao, L.; Feng, W.; Li, F. A Cyanine-Modified Nanosystem for in Vivo Upconversion Luminescence Bioimaging of Methylmercury. J. Am. Chem. Soc. 2013, 135 (26), 9869-9876. (7) Peng, J.; Xu, W.; Teoh, C. L.; Han, S.; Kim, B.; Samanta, A.; Er, J. C.; Wang, L.; Yuan, L.; Liu, X.; Chang, Y.-T. High-Efficiency in Vitro and in Vivo Detection of Zn2 by Dye-Assembled Upconversion Nanoparticles. J. Am. Chem. Soc. 2015, 137 (6), 2336-2342. (8) Deng, R.; Xie, X.; Vendrell, M.; Chang, Y.-T.; Liu, X. Intracellular Glutathione Detection Using MnO2-Nanosheet-Modified Upconversion Nanoparticles. J. Am. Chem. Soc. 2011, 133 (50), 20168-20171. (9) Liu, Y.; Zhou, S.; Tu, D.; Chen, Z.; Huang, M.; Zhu, H.; Ma, E.; Chen, X. Amine-Functionalized Lanthanide-Doped Zirconia Nanoparticles: Optical Spectroscopy, Time-Resolved Fluorescence Resonance Energy Transfer Biodetection, and Targeted Imaging. J. Am. Chem. Soc. 2012, 134 (36), 15083-15090. (10) Li, Z.; Lv, S.; Wang, Y.; Chen, S.; Liu, Z. Construction of LRET-Based Nanoprobe Using Upconversion Nanoparticles with Confined Emitters and Bared Surface as Luminophore. J. Am. Chem. Soc. 2015, 137 (9), 3421-3427. (11) Xiao, Y.; Zeng, L.; Xia, T.; Wu, Z.; Liu, Z. Construction of an Upconversion Nanoprobe with Few-Atom Silver Nanoclusters as the Energy Acceptor. Angew. Chem. Int. Ed. 2015, 54 (18), 5323-5327. (12) Chen, Z.; Liu, Z.; Li, Z.; Ju, E.; Gao, N.; Zhou, L.; Ren, J.; Qu, X. Upconversion nanoprobes for efficiently in vitro imaging reactive oxygen species and in vivo diagnosing rheumatoid arthritis. Biomaterials 2015, 39, 15-22. (13) Zhang, J.; Li, B.; Zhang, L.; Jiang, H. An optical sensor for Cu(ii) detection with upconverting luminescent nanoparticles as an excitation source. Chem. Commun. 2012, 48 (40), 4860-4862. (14) Ni, J. K.; Shan, C. X.; Li, B.; Zhang, L. M.; Ma, H. P.; Luo, Y. S.; Song, H. Assembling of a functional cyclodextrin-decorated upconversion luminescence nanoplatform for cysteine-sensing. Chem. Commun. 2015, 51 (74), 14054-14056.

3. 毕业设计（论文）进程安排

2018.1.2-2018.3.1 查阅资料，完成开题报告和任务书 2018.3.2-2018.3.10 确定方案，学习实验过程和操作 2018.3.11-2018.4.30 独立实验，合成产品并进行结构表征 2018.5.1-2018.5.30 数据整理，书写论文，制作PPT 2018.5.30- 修改论文并完成答辩