1. 毕业设计（论文）的内容和要求

基于荧光技术的检测方法通常被称为荧光光谱测量法，分子荧光法是其理论基础。

荧光技术检测二氧化硫的原理很简单，二氧化硫在激发光源的激发下就会产生荧光，利用浓度与探针产生的荧光强度之间的线性关系进行定量的分析，运用荧光光谱的形状和荧光峰值对应的波长进行定性的分析。

利用荧光方法检测样品中二氧化硫浓度具有灵敏度高、重复性好、取样简单、等诸多优点。

2. 参考文献

1. Li, L., Rose, P. Moore, P. K. Hydrogen sulfide and cell signaling. Annu. Rev. Pharmacol. Toxicol. 51, 169-187 (2011). 2. Kamoun, P. Endogenous production of hydrogen sulfide in mammals. Amino Acids 26, 243-254 (2004). 3. Chiku, T. et al. H2S biogenesis by human cystathionine gamma-lyase leads to the novel sulfur metabolites lanthionine and homolanthionine and is responsive to the grade of hyperhomocysteinemia. J. Biol. Chem. 284, 11601-11612 (2009). 4. Singh, S., Padovani, D., Leslie, R. A., Chiku, T. Banerjee, R. Relative contributions of cystathionine beta-synthase and gamma-cystathionase to H2S biogenesis via alternative trans-sulfuration reactions. J. Biol. Chem. 284, 22457-22466 (2009). 5. Shibuya, N. et al. 3-mercaptopyruvate sulfurtransferase produces hydrogen sulfide and bound sulfane sulfur in the brain. Antioxid. Redox Signal. 11, 703-714 (2009). 6. Mustafa, A. K. et al. Hydrogen sulfide as endothelium-derived hyperpolarizing factor sulfhydrates potassium channels. Circ. Res. 109, 1259-1268 (2011). 7. Yang, G. et al. H2S as a physiologic vasorelaxant: Hypertension in mice with deletion of cystathionine γ-lyase. Science 322, 587-590 (2008). 8. Kimura, H. Hydrogen sulfide as a neuromodulator. Mol. Neurobiol. 26, 13-19 (2002). 9. Olson, K. R. et al. Hydrogen sulfide as an oxygen sensor/transducer in vertebrate hypoxic vasoconstriction and hypoxic vasodilation. J. Exp. Biol. 209, 4011-4023 (2006). 10. Peng, Y. J. et al. H2S mediates O2 sensing in the carotid body. Proc. Natl. Acad. Sci. USA 107, 10719-10724 (2010). 11. Papapetropoulos, A. et al. Hydrogen sulfide is an endogenous stimulator of angiogenesis. Proc. Natl. Acad. Sci. USA 106, 21972-21977 (2009). 12. Szab#243;, C. Hydrogen sulphide and its therapeutic potential. Nat. Rev. Drug Discov. 6, 917-935 (2007). 13. Kamoun, P., Belardinelli, M. C., Chabli, A., Lallouchi, K. Chadefaux, V. B. Endogenous hydrogen sulfide overproduction in Down syndrome. Am. J. Med. Genet. A 116A, 310-311 (2003). 14. Eto, K., Asada. T., Arima, K., Makifuchi, T. Kimura, H. Brain hydrogen sulfide is severely decreased in Alzheimer#8217;s disease. Biochem. Biophys. Res. Commun. 293, 1485-1488 (2002). 15. Fiorucci, S. et al. The third gas: H2S regulates perfusion pressure in both the isolated and perfused normal rat liver and in cirrhosis. Hepatology 42, 539-548 (2005). 16. Jha, S., Calvert, J. W., Duranski, M. R., Ramachandran, A. Lefer, D. J. Hydrogen sulfide attenuates hepatic ischemia-reperfusion injury: role of antioxidant and antiapoptotic signaling. Am. J. Physiol. Heart Circ. Physiol. 295, H801-H806 (2008). 17. Suzuki, K. et al. Hydrogen sulfide replacement therapy protects the vascular endothelium in hyperglycemia by preserving mitochondrial function. Proc. Natl Acad. Sci. USA 108, 13829-13834 (2011). 18. Schiavon, G., Zotti, G., Toniolo, R. Bontempelli, G. Electrochemical detection of trace hydrogen sulfide in gaseous samples by porous silver electrodes supported on ion-exchange membranes (solid polymer electrolytes). Anal. Chem. 67, 318-323 (1995).

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2017.1.2-2017.3.1 查阅资料，完成开题报告和任务书 2017.3.2-2017.3.10 确定方案，学习实验过程和操作 2017.3.11-2017.4.30 独立实验，合成产品并进行结构表征 2017.5.1-2017.5.30 数据整理，书写论文，制作PPT 2017.5.30- 修改论文并完成答辩