1. 毕业设计（论文）的内容和要求

1、毕业论文主要内容 硝基还原酶（nitroreductase, ntr）属于黄素酶，通常含有黄素单核苷酸或黄素腺嘌呤二核苷酸单元，它能够利用烟酰胺嘌呤二核苷酸（nicotinamide adenine dinucleotide phosphate, nadph）以及烟酰胺腺嘌呤二核苷酸磷酸（nicotinamide adenine dinucleotid, nadh）作为辅酶，实现对多种硝基化合物的还原代谢，生成羟胺或氨基。

因此在电子供体如nadph/nadh的存在下，ntr可将芳香族硝基化合物有效还原为对应的氨基化合物。

该种还原行为可用来设计含硝基的荧光探针对实体瘤细胞的缺氧状况进行实时检测，直接指示肿瘤细胞的存在，具有十分重要的意义。

2. 参考文献

[1]Li Y, Sun Y, Li J, Su Q, Yuan W, Dai Y, Han C, Wang Q, Feng W, Li F. Ultrasensitive near-infrared fluorescence-enhanced probe for in vivo nitroreductase imaging. J. Am. Chem. Soc. 2015, 137: 6407-16. [2]High selectivity imaging of nitroreductase using a near-infrared fluorescence probe in hypoxic tumor.Xu K, Wang F, Pan X, Liu R, Ma J, Kong F, Tang B.Chem. Commun. (Camb.) 2013, 49: 2554-6. [3]A novel near-infrared fluorescent probe for selectively sensing nitroreductase (NTR) in an aqueous medium.Shi Y, Zhang S, Zhang X.Analyst 2013, 138: 1952-5. [4]A cresyl violet-based fluorescent off-on probe for the detection and imaging of hypoxia and nitroreductase in living organisms.Wan QQ, Gao XH, He XY, Chen SM, Song YC, Gong QY, Li XH, Ma HM.Chem Asian J 2014, 9: 2058-62. [5]Efficient Two-Photon Fluorescent Probe for Nitroreductase Detection and Hypoxia Imaging in Tumor Cells and Tissues.Zhang J, Liu HW, Hu XX, Li J, Liang LH, Zhang XB, Tan W.Anal. Chem. 2015, 87: 11832-9. [6]Garakyaraghi S, McCusker CE, Khan S, Koutnik P, Bui AT, Castellano FN. Enhancing the Visible-Light Absorption and Excited-State Properties of Cu(I) MLCT Excited States. Inorg Chem 2018, 57: 2296-2307. [7]Transient absorption dynamics of sterically congested Cu(I) MLCT excited states.Garakyaraghi S, Danilov EO, McCusker CE, Castellano FN.J Phys Chem A 2015, 119: 3181-93. [8]Ultrafast excited-state dynamics of copper(I) complexes.Iwamura M, Takeuchi S, Tahara T.Acc. Chem. Res. 2015, 48: 782-91. [9]Bidirectional "ping-pong" energy transfer and 3000-fold lifetime enhancement in a Re(I) charge transfer complex.Yarnell JE, Deaton JC, McCusker CE, Castellano FN.Inorg Chem 2011, 50: 7820-30. [10]Wang P, Wu J, Su P, Xu C, Ge Y, Liu D, Liu W, Tang Y. Fluorescence on-off-on peptide-based chemosensor for the selective detection of Cuand Sand its application in living cell bioimaging. Dalton Trans 2016, 45: 16246-16254. [11]A novel peptide-based fluorescence chemosensor for selective imaging of hydrogen sulfide both in living cells and zebrafish.Wang P, Wu J, Di C, Zhou R, Zhang H, Su P, Xu C, Zhou P, Ge Y, Liu D, Liu W, Tang Y.Biosens Bioelectron 2017, 92: 602-609. [12]A peptide-based fluorescent chemosensor for measuring cadmium ions in aqueous solutions and live cells.Wang P, Wu J, Liu L, Zhou P, Ge Y, Liu D, Liu W, Tang Y.Dalton Trans 2015, 44: 18057-64. [13]A reversible fluorescence chemosensor for sequentially quantitative monitoring copper and sulfide in living cells.Meng Q, Zhang R, Jia H, Gao X, Wang C, Shi Y, Everest-Dass AV, Zhang Z.Talanta 2015, 143: 294-301. [14]Zhang H, Liu R, Liu J, Li L, Wang P, Yao SQ, Xu Z, Sun H. A minimalist fluorescent probe for differentiating Cys, Hcy and GSH in live cells. Chem Sci 2016, 7: 256-260. [15]A fluorescent probe for the efficient discrimination of Cys, Hcy and GSH based on different cascade reactions.Li Y, Liu W, Zhang P, Zhang H, Wu J, Ge J, Wang P.Biosens Bioelectron 2017, 90: 117-124. [16]A dual-selective fluorescent probe for GSH and Cys detection: Emission and pH dependent selectivity.Tang Y, Jin L, Yin B.Anal. Chim. Acta 2017, 993: 87-95. [17]A fluorescent probe for simultaneous discrimination of GSH and Cys/Hcy in human serum samples via distinctly-separated emissions with independent excitations.Hu Q, Yu C, Xia X, Zeng F, Wu S.Biosens Bioelectron 2016, 81: 341-348. [18]Li L, Zhang CW, Ge J, Qian L, Chai BH, Zhu Q, Lee JS, Lim KL, Yao SQ. A Small-Molecule Probe for Selective Profiling and Imaging of Monoamine Oxidase B Activities in Models of Parkinson s Disease. Angew. Chem. Int. Ed. Engl. 2015, 54: 10821-5. [19]A sensitive two-photon probe to selectively detect monoamine oxidase B activity in Parkinson's disease models.Li L, Zhang CW, Chen GY, Zhu B, Chai C, Xu QH, Tan EK, Zhu Q, Lim KL, Yao SQ. Nat Commun 2014, 5: 3276. [20]The adenosine receptor affinities and monoamine oxidase B inhibitory properties of sulfanylphthalimide analogues.Van der Walt MM, Terre'Blanche G, Petzer A, Petzer JP.Bioorg. Chem. 2015, 59: 117-23. [21]Oxazolopyridines and thiazolopyridines as monoamine oxidase B inhibitors for the treatment of Parkinson's disease.Park HR, Kim J, Kim T, Jo S, Yeom M, Moon B, Choo IH, Lee J, Lim EJ, Park KD, Min SJ, Nam G, Keum G, Lee CJ, Choo H.Bioorg. Med. Chem. 2013, 21: 5480-7. 1-[2-(4-Benzyloxyphenoxy)Ethyl]Imidazole inhibits monoamine oxidase B and protects against neuronal loss and behavioral impairment in rodent models of Parkinson's disease.Chung JY, Lee JW, Ryu CH, Min HK, Yoon YJ, Lim MJ, Park CH.J. Neurosci. Res. 2015, 93: 1267-78.

3. 毕业设计（论文）进程安排

起讫日期 设计（论文）各阶段工作内容 备 注 2018.2.23-2018.3.2 课题任务书、开题报告、文献综述与翻译 2018.3.3-2018.3.7 实验方案设计与确认，实验材料准备 2018.3.8-2018.5.4 进行实验 2018.5.5-2018.5.10 实验，中期答辩 2018.5.11-2018.5.30 实验，整理实验数据，撰写毕业论文 论文初稿 2018.6.1-2018.6.5 修改论文，准备答辩材料 论文二稿 2018.6.6-2018.6.9 修改论文 定稿 2018.6.10 论文答辩