1. 毕业设计（论文）的内容和要求

1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-coa含量对菌体产油脂情况的影响，撰写文献综述。

3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]．孙美莉，刘虎虎，邬文嘉，任路静，黄和，纪晓俊. 代谢工程改造酵母生产多不饱和脂肪酸的研究进展[J]. 化工进展，2016,35(3). [2]．Vineet Choudhary, Nicolas Jacquier, Roger Schneiter.The topology of the triacylglycerol synthesizing enzyme Lro1 indicates that neutral lipids can be produced within the luminal compartment of the endoplasmatic reticulum: Implications for the biogenesis of lipid droplets[J].Communicative amp; Integrative Biology, 2011, 4(6):781 -784. [3]．Kyung Ok Yu, Ju Jung, Ahmad Bazli Ramzi, et al. Development of a Saccharomyces cerevisiae strain for increasing the accumulation of triacylglycerol as a microbial oil feedstock for biodiesel production using glycerol as a substrate.[J]. Biotechnology amp; Bioengineering, 2013, 110(1):343-7. [4]．Yeshitila Asteraye Tsigie, Chun-Yuan Wang, Chi-Thanh Truong.Lipid production from Yarrowia lipolytica Po1g grown in sugarcane bagasse hydrolysate[J]. Bioresour. Technol, 2012, 102, 9216#8211;9222. [5]．Lu-Te Chuang, Dzi-Chi Chen, Jean-Marc Nicaud.Coexpression of heterologous desaturase genes in Yarrowia lipolytica[J]. New Biotechnology. 2010, 27: 277-282. [6]．Jean-Marc Nicaud, Annick Le Clainche, Marie-Therese Le Dall,et al. Yarrowia lipolytica,a yeast model for the genetic studies of hydroxy fatty acids biotransformati- -on into lactone[J]. Journal of Molecular Catalysis B: Enzymatic 5,1998,175-181 [7]．P. Fickers, M.T. Le Dall, C. Gaillardin. New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica[J].Journal of Microbiological Methods, 2003,727-737. [8]．B.Sauer. Functional expression of the cre-lox site-specific recombination system in the yeast Saccharomyces cerevisiae.[J]. Molecular amp; Cellular Biology, 1987, 7(6):2087. [9]．Jean-Marc Nicaud, Annick Le Clainche, Marie-Therese Le Dall,et al. Yarrowia lipolytica,a yeast model for the genetic studies of hydroxy fatty acids biotransformati- -on into lactone[J]. Journal of Molecular Catalysis B: Enzymatic 5,1998,175-181. [10]．Tigran V. Yuzbashe, Evgeniya Y. Yuzbasheva, Tatiana I. Sobolevskaya, et al. Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica[J]. Biotechnology amp; Bioengineering, 2010, 107(4):673-682. [11]．Ramdane Haddouche, Yves Poirier, Syndie Delessert, et al. Engineering polyhydroxyalkanoate content and monomer composition in the oleaginous yeast Yarrowia lipolytica by modifying the szlig;-oxidation multifunctional protein[J]. Applied Microbiology amp; Biotechnology, 2011, 91(5):1327. [12]．A. Beopoulos, J. Verbeke, F. Bordes, et al. Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica[J]. Applied Microbiology amp; Biotechnology, 2014, 98(1):251-262. [13]．Cuijuan Gao, Xiaofeng Yang, Huaimin Wang, et al. Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica[J]. Biotechnology for Biofuels, 2016, 9(1):179. [14]．Zhiyong Cui, Cuijuan Gao, Jiaojiao Li, et al. Engineering of unconventional yeast Yarrowia lipolytica, for efficient succinic acid production from glycerol at low pH[J]. Metabolic Engineering, 2017, 42:126-133. [15]．周杨, 朱建国, 唐小春,等. 表达Cre重组酶Cre-pCEP4载体的构建及其在Cre/loxP重组系统中的应用[J]. 吉林大学学报(医学版), 2011, 37(2):196-201. [16]．K.Mlikova, Acyl-CoA oxidase, a key step for lipid accumulation in the yeast Yarrowia lipolytica. J. Mol. Catal B-Enzym. 2004, 28, 81-85.

3. 毕业设计（论文）进程安排

2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写