1. 毕业设计（论文）的内容和要求

1．文献查阅掌握文献查阅的一般方法，学会使用计算机在中国期刊网、维普数据库、超星数字图书馆等中文数据库、ca、sci等外文数据库检索资源上查阅有关文献。

2．文献阅读及综述阅读与课题相关的外文及中文文献，了解解脂耶氏酵母胞质中乙酰-coa含量对菌体产油脂情况的影响，撰写文献综述。

3．明确实验任务，拟定实验方案根据所查阅文献的内容，确定实验内容及方案，拟定科学可行的研究计划。

2. 参考文献

[1]．Ali Abghari, Shulin Chen. Yarrowia lipolytica as an Oleaginous Cell Factory Platform for Production of Fatty Acid-Based Biofuel and Bioproducts[J]. Frontiers in Energy Research, 2014, 2:21. [2]．Huhu Liu, Xiaojun Ji, He Huang. Biotechnological applications of Yarrowia lipolytica:Past, present and future[J]. Biotechnology Advances, 2015 33(8):1522-154 6. [3]．Karin Athenstaedt. YALI0E32769g (DGA1) and YALI0E16797g (LRO1) encode major triacylglycerol synthases of the oleaginous yeastYarrowia lipolytica[J]. Biochimica et Biophysica Acta, 2011, 1811(10):587-596. [4]．Vineet Choudhary, Nicolas Jacquier, Roger Schneiter.The topology of the triacylglycerol synthesizing enzyme Lro1 indicates that neutral lipids can be produced within the luminal compartment of the endoplasmatic reticulum: Implications for the biogenesis of lipid droplets[J].Communicative amp; Integrative Biology, 2011, 4(6):781 -784. [5]．Seraphim Papanikolaou1 , George Aggelis. Lipids of oleaginous yeasts.PartI: Biochemistry of single cell oil production[J].Eur. J. Lipid Sci. Technol. 2011,113, 1031-1051. [6]．Seraphim Papanikolaou , George Aggelis.Lipids of oleaginous yeasts. Part II: Technology and potential applications[J]. Eur. J. Lipid Sci. Technol. 2011,113:1052-1 073. [7]．Lu-Te Chuang, Dzi-Chi Chen, Jean-Marc Nicaud.Coexpression of heterologous desaturase genes in Yarrowia lipolytica[J]. New Biotechnology. 2010, 27: 277-282. [8]．Peng Xu, Kangjian Qiao, Woo Suk Ahna.Engineering Yarrowia lipolytica as a platform for synthesis of drop-in transportation fuels and oleochemicals[J]. Proc Natl Acad Sci. 2016, 113(39):10848-53. [9]．Jean-Marc Nicaud, Annick Le Clainche, Marie-Therese Le Dall,et al. Yarrowia lipolytica,a yeast model for the genetic studies of hydroxy fatty acids biotransformati- -on into lactone[J]. Journal of Molecular Catalysis B: Enzymatic 5,1998,175-181. [10]．P. Fickers, M.T. Le Dall, C. Gaillardin. New disruption cassettes for rapid gene disruption and marker rescue in the yeast Yarrowia lipolytica[J].Journal of Microbiological Methods, 2003,727-737. [11]．Jonathan Verbeke, Athanasios Beopoulos, Jean-Marc Nicaud. Efficient homologous recombination with short length flanking fragments in Ku70 deficient Yarrowia lipolytica strains[J]. Biotechnol Lett (2013) 35:571-576. [12]．Jean-Marc Nicaud, Annick Le Clainche, Marie-Therese Le Dall,et al. Yarrowia lipolytica, a yeast model for the genetic studies of hydroxy fatty acids biotransformation into lactones[J]. Journal of Molecular Catalysis B Enzymatic, 1998, 5(1-4):175-181. [13]．Tigran V. Yuzbashev, Evgeniya Y. Yuzbasheva, Tatiana I. Sobolevskaya, , et al. Production of succinic acid at low pH by a recombinant strain of the aerobic yeast Yarrowia lipolytica[J]. Biotechnology amp; Bioengineering, 2010, 107(4):673-682. [14]．A.Beopoulos ,J.Verbeke, F,Bordes, et al. Metabolic engineering for ricinoleic acid production in the oleaginous yeast Yarrowia lipolytica[J]. Applied Microbiology amp; Biotechnology, 2014, 98(1):251-262. [15]．Cuijuan Gao,Xiaofeng Yang,Huaimin Wang, et al. Robust succinic acid production from crude glycerol using engineered Yarrowia lipolytica[J]. Biotechnology for Biofuels, 2016, 9(1):179. Zhiyong Cui , Cuijuan Gao , Jiaojiao Lia ,et al. Engineering of unconventional yeast Yarrowia lipolytica, for efficient succinic acid production from glycerol at low pH[J]. Metabolic Engineering, 2017, 42:126-133.

3. 毕业设计（论文）进程安排

2.10-3.1 确定毕业设计的课题及相关资料的查找 3.2-3.10 实验方案的设计 3.10-3.20 实验材料的准备 3.21-5.15 准备分子实验 5.15-6.5 实验数据处理与结果分析 5.20-6.10 毕业论文书写