海洋细菌Pedobacter sp.NJ-02中卡拉胶酶重组表达及性质研究任务书
2020-06-24 19:52:25
1. 毕业设计(论文)的内容和要求
对海洋细菌Pedobacter sp.NJ-02中卡拉胶酶进行重组表达,并研究其酶学性质及产物的分布情况。
2. 参考文献
[1]纪明候.海藻化学[M].北京:科学出版社,1997:117-187. [2]Sarwar G,Matayoshi S,Oda H.Purification of a kapprcarageenase from marine Cytophaga species [J].Microbiol Immunol,1987,31(9):869877. [3]Araki T,Higashimoto Y,Morishita T.Purification and chara-terization of kapprcarragenase from a marine bacterium /ibriosp.CA-1004[J].Fish Sci,1999,(6):937-942. [4]Yamada T,Ogamo A,Saito T,ct al.Preparation and ant- HIVactivity of louwmolecularweight carragenans and their slfatedderivatives [J].Carbolhyd Polym,197,32(l):51-55. [5]Mou HJ,Jiang XL,Gan HS.A -caragemn derivcd oligsaccharide prepared by enzymatic degradation containing ant-tumor activity [J].JAppl Phycol,2003,15(4):297-303.
3. 毕业设计(论文)进程安排
2017.12-2018.3 调研文献资料,确定方案; 2018.4-2018.5 开展实验,完成酶的重组表达,酶学性质研究及产物分析; 2018.5-2018.6 撰写论文,准备答辩。